Characterization of the fecal microbiome during neonatal and early pediatric development in puppies
| Autor: | Blake C. Guard, Jan S. Suchodolski, Jörg M. Steiner, Sylvie Chastant-Maillard, Claire Mariani, Hanna Mila |
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| Přispěvatelé: | Texas A&M University System, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Royal Canin, Chastant-Maillard, Sylvie |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
intestinal microbiota dog puppy Time Factors Physiology lcsh:Medicine Artificial Gene Amplification and Extension Pathology and Laboratory Medicine Biochemistry Polymerase Chain Reaction Feces Puppy Antibiotics RNA Ribosomal 16S Medicine and Health Sciences lcsh:Science Phylogeny Mammals Multidisciplinary biology Ecology Antimicrobials Microbiota Microbiology and Parasitology microbiote fécal Drugs Genomics fusobacterium Microbiologie et Parasitologie 3. Good health Bacterial Pathogens Nucleic acids [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Ribosomal RNA Medical Microbiology Vertebrates matière fécale Pathogens développement néonatal Research Article DNA Bacterial Cell biology Cellular structures and organelles Ecological Metrics Firmicutes 030106 microbiology Microbial Genomics Real-Time Polymerase Chain Reaction Research and Analysis Methods Microbiology Fusobacteria 03 medical and health sciences Dogs biology.animal Gut bacteria Microbial Control Genetics Animals Microbiome Non-coding RNA Molecular Biology Techniques Molecular Biology Microbial Pathogens Pharmacology Clostridium Bacteria Bacteroidetes Neonatal survival lcsh:R Ecology and Environmental Sciences Gut Bacteria Organisms Biology and Life Sciences Neonates Species Diversity Sequence Analysis DNA biology.organism_classification 030104 developmental biology Animals Newborn firmicute chien Amniotes RNA lcsh:Q Ribosomes Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (4), 17 p. ⟨10.1371/journal.pone.0175718⟩ Plos One 4 (12), 17 p.. (2017) PLoS ONE, Vol 12, Iss 4, p e0175718 (2017) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0175718⟩ |
| Popis: | International audience; Limited information is available describing the development of the neonatal fecal microbiome in dogs. Feces from puppies were collected at 2, 21, 42, and 56 days after birth. Feces were also collected from the puppies’ mothers at a single time point within 24 hours after parturition. DNA was extracted from fecal samples and 454-pyrosequencing was used to profile 16S rRNA genes. Species richness continued to increase significantly from 2 days of age until 42 days of age in puppies. Furthermore, microbial communities clustered separately from each other at 2, 21, and 42 days of age. The microbial communities belonging to dams clustered separately from that of puppies at any given time point. Major phylogenetic changes were noted at all taxonomic levels with the most profound changes being a shift from primarily Firmicutes in puppies at 2 days of age to a co-dominance of Bacteroidetes, Fusobacteria, and Firmicutes by 21 days of age. Further studies are needed to elucidate the relationship between puppy microbiota development, physiological growth, neonatal survival, and morbidity. |
| Databáze: | OpenAIRE |
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