Defects in Protein Glycosylation Cause SHO1-Dependent Activation of a STE12 Signaling Pathway in Yeast
| Autor: | George F. Sprague, Yoshifumi Jigami, Janet Schultz, Joe Horecka, B. J. Stevenson, Paul J. Cullen |
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| Rok vydání: | 2000 |
| Předmět: |
Glycosylation
Saccharomyces cerevisiae Proteins Transcription Genetic Recombinant Fusion Proteins Mutant Saccharomyces cerevisiae Mannose Biology Pheromones Fungal Proteins chemistry.chemical_compound Cell Wall Transcription (biology) Genetics Promoter Regions Genetic Transcription factor Protein Kinase C Fungal protein Membrane Glycoproteins Genetic Complementation Test Membrane Proteins biology.organism_classification chemistry Biochemistry Mutation Mitogen-Activated Protein Kinases Signal transduction Signal Transduction Transcription Factors Research Article |
| Zdroj: | Genetics. 155:1005-1018 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/155.3.1005 |
| Popis: | In haploid Saccharomyces cerevisiae, mating occurs by activation of the pheromone response pathway. A genetic selection for mutants that activate this pathway uncovered a class of mutants defective in cell wall integrity. Partial loss-of-function alleles of PGI1, PMI40, PSA1, DPM1, ALG1, MNN10, SPT14, and OCH1, genes required for mannose utilization and protein glycosylation, activated a pheromone-response-pathway-dependent reporter (FUS1) in cells lacking a basal signal (ste4). Pathway activation was suppressed by the addition of mannose to hexose isomerase mutants pgi1-101 and pmi40-101, which bypassed the requirement for mannose biosynthesis in these mutants. Pathway activation was also suppressed in dpm1-101 mutants by plasmids that contained RER2 or PSA1, which produce the substrates for Dpm1. Activation of FUS1 transcription in the mannose utilization/protein glycosylation mutants required some but not all proteins from three different signaling pathways: the pheromone response, invasive growth, and HOG pathways. We specifically suggest that a Sho1 → Ste20/Ste50 → Ste11 → Ste7 → Kss1 → Ste12 pathway is responsible for activation of FUS1 transcription in these mutants. Because loss of pheromone response pathway components leads to a synthetic growth defect in mannose utilization/protein glycosylation mutants, we suggest that the Sho1 → Ste12 pathway contributes to maintenance of cell wall integrity in vegetative cells. |
| Databáze: | OpenAIRE |
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