Interleukin-1 beta induces cytosolic PLA2 in parallel with prostaglandin E2 in rheumatoid synovial fibroblasts
| Autor: | Keren I. Hulkower, Wayne Levin, Celia M. Anderson, William C. Hope, John W. Coffey, David Robert Bolin, Ting Chen, Douglas W. Morgan |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Immunology Toxicology Dinoprostone Phospholipases A Arthritis Rheumatoid Phospholipase A2 Cytosol Internal medicine medicine Humans Pharmacology (medical) Prostaglandin E2 Fibroblast Cells Cultured Pharmacology chemistry.chemical_classification biology business.industry Synovial Membrane Fibroblasts medicine.disease Molecular biology In vitro Molecular Weight Phospholipases A2 Enzyme medicine.anatomical_structure Cytokine Endocrinology chemistry Rheumatoid arthritis Enzyme Induction biology.protein lipids (amino acids peptides and proteins) business medicine.drug Interleukin-1 |
| Zdroj: | Agents and actions. |
| ISSN: | 0065-4299 |
| Popis: | We investigated the temporal relationship between the increase in enzymatic activity and protein of a high molecular weight (100 kDa), cytosolic PLA2 (cPLA2) in interleukin-1 beta (IL-1 beta)-treated rheumatoid synovial fibroblasts (RSF). Both of these responses increased according to a similar time-course which correlates with PGE2 production by these cells. In contrast, 14 kDa, secreted PLA2 (sPLA2), which was also produced by RSF, was not affected by IL-1 beta treatment. These findings support that an augmentation of CPLA2 activity, caused by an induction of cPLA2 protein, rather than sPLA2, is temporally associated with increased PGE2 production in IL-1 beta-treated RSF. |
| Databáze: | OpenAIRE |
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