Introgression mapping of genes for winter hardiness and frost tolerance transferred from Festuca arundinacea into Lolium multiflorum
| Autor: | Dagmara Gasior, Michael W. Humphreys, Zbigniew Zwierzykowski, Marcin Rapacz, E. Zwierzykowska, Arkadiusz Kosmala, Michal W. Luczak |
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| Rok vydání: | 2007 |
| Předmět: |
Festuca
biology Genotype Adaptation Biological Gene Transfer Techniques Introgression Chromosome Mapping Lolium multiflorum Breeding biology.organism_classification Plants Genetically Modified Lolium Botany Genetics Festuca pratensis Seasons Hardiness (plants) Molecular Biology Festuca arundinacea Genetics (clinical) In Situ Hybridization Fluorescence Biotechnology Hybrid |
| Zdroj: | The Journal of heredity. 98(4) |
| ISSN: | 0022-1503 |
| Popis: | Genes for winter hardiness and frost tolerance were introgressed from Festuca arundinacea into winter-sensitive Lolium multiflorum. Two partly fertile, pentaploid (2n = 5x = 35) F(1) hybrids F. arundinacea (2n = 6x = 42) x L. multiflorum (2n = 4x = 28) were generated and backcrossed twice onto L. multiflorum (2x). The backcross 1 (BC(1)) and backcross 2 (BC(2)) plants were preselected for high vigor and good fertility, and subsequently, a total of 83 BC(2) plants were selected for winter hardiness after 2 Polish winters and by simulated freezing tests. Genomic in situ hybridization (GISH) was performed on 6 winter-hardy plants selected after the first winter and shown to be significantly (P0.05) more frost tolerant than the L. multiflorum control. Among the analyzed BC(2) winter survivors, only diploid (2n = 2x = 14) plants were found. Five plants carried 13 intact L. multiflorum chromosomes and 1 L. multiflorum chromosome with a single introgressed F. arundinacea terminal chromosome segment. The sixth BC(2) winter survivor appeared to be Lolium without any Festuca introgression capable of detection by GISH. A combined GISH and fluorescence in situ hybridization analysis with rDNA probes of the most winter-hardy (after 2 winters) and frost-tolerant BC(2) plant revealed the location of an F. arundinacea introgression on the nonsatellite arm of L. multiflorum chromosome 2, the same chromosome location reported previously as a site for frost tolerance genes in the diploid and winter-hardy species Festuca pratensis. |
| Databáze: | OpenAIRE |
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