Structural characterization of MAPLE deposited lipase biofilm
| Autor: | Patrizio Massoli, Luciano Rosario Maria Vicari, Esther Fanelli, Antonio Aronne, Giovanni Ausanio, R. Calabria, Valeria Califano, Francesco Bloisi |
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| Přispěvatelé: | Aronne, Antonio, Ausanio, Giovanni, Bloisi, Francesco, Raffaela, Calabria, Valeria, Califano, Fanelli, Esther, Patrizio, Massoli, Vicari, LUCIANO ROSARIO MARIA |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
MAPLE Analytical chemistry General Physics and Astronomy engineering.material Matrix-Assisted Pulsed Laser Evaporation (MAPLE) Native state Deposition (phase transition) Lipase Fourier transform infrared spectroscopy Maple Laser ablation biology Biofilm Surfaces and Interfaces General Chemistry Transesterification Condensed Matter Physics Evaporation (deposition) Surfaces Coatings and Films Chemical engineering biology.protein engineering Biodiesel |
| Zdroj: | Applied surface science 320 (2014): 524–530. doi:10.1016/j.apsusc.2014.09.112 info:cnr-pdr/source/autori:A. Aronne a, G. Ausanio b, F. Bloisi b, R. Calabria c, V. Califano c, E. Fanelli a, P. Massoli, L.R.M. Vicari b/titolo:Structural characterization of MAPLE deposited lipase biofilm/doi:10.1016%2Fj.apsusc.2014.09.112/rivista:Applied surface science/anno:2014/pagina_da:524/pagina_a:530/intervallo_pagine:524–530/volume:320 |
| Popis: | Lipases (triacylglycerol ester hydrolases) are enzymes used in several industrial applications. Enzymes immobilization can be used to address key issues limiting widespread application at industrial level. Immobilization efficiency is related to the ability to preserve the native conformation of the enzyme. MAPLE (Matrix Assisted Pulsed Laser Evaporation) technique, a laser deposition procedure for treating organic/polymeric/biomaterials, was applied for the deposition of lipase enzyme in an ice matrix, using near infrared laser radiation. Microscopy analysis showed that the deposition occurred in micrometric and submicrometric clusters with a wide size distribution. AFM imaging showed that inter-cluster regions are uniformly covered with smaller aggregates of nanometric size. Fourier transform infrared spectroscopy was used for both recognizing the deposited material and analyzing its secondary structure. Results showed that the protein underwent reversible self-association during the deposition process. Actually, preliminary tests of MAPLE deposited lipase used for soybean oil transesterification with isopropyl alcohol followed by gas chromatography–mass spectrometry gave results consistent with undamaged deposition of lipase. |
| Databáze: | OpenAIRE |
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