The effects of aminosalicylic acid derivatives on nitric oxide in a cell-free system
| Autor: | SJ Middleton, P. D. Reynolds, JO Hunter, M Shorthouse |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Aminosalicylic acid
Inorganic chemistry Anti-Inflammatory Agents Nitric Oxide Nitric oxide Cell-free system chemistry.chemical_compound Gastrointestinal Agents Sulfapyridine medicine Pharmacology (medical) Nitrite IC50 Dose-Response Relationship Drug Hepatology Nitric oxide binding Anti-Inflammatory Agents Non-Steroidal Gastroenterology Sulfasalazine Aminosalicylic Acids chemistry Mechanism of action Colitis Ulcerative Sodium nitroprusside medicine.symptom Polarography medicine.drug Nuclear chemistry |
| Zdroj: | Alimentary Pharmacology & Therapeutics. 9:491-495 |
| ISSN: | 1365-2036 0269-2813 |
| DOI: | 10.1111/j.1365-2036.1995.tb00411.x |
| Popis: | SUMMARY Aims and Methods: To determine the effect of aminosalicylic acid derivatives on the concentration of nitric oxide produced in a cell-free system, by the use of a sensitive and specific polarographic meter. Results: The aminosalicylic acid derivatives 3-ASA (IC50 100 μm), 4-ASA (IC50 350 μm) and 5-ASA (IC50 5 μm) all decreased the nitric oxide signal. These drugs had a similar inhibitory effect on the formation in vitro of nitrite from sodium nitroprusside (IC50 200 μm, 500 μm and 100 μm, respectively). Sulphasalazine (31.1 ± 5% decrease in signal at 1 mm) was less effective than 5-ASA, but sulphapyridine, N-acetyl 5-ASA, indomethacin and hydrocortisone produced no decrease in nitric oxide signal at all. Conclusions: Nitric oxide binding may be part of the mechanism by which ASA derivatives exert their therapeutic effect, and this work suggests that it may be an important factor in the pathogenesis of ulcerative colitis. |
| Databáze: | OpenAIRE |
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