2-Ketogluconic Acid Production and Phosphate Solubilization by Enterobacter intermedium
| Autor: | Yo Sup Rim, Keun Hyung Park, Tae-Hwan Kim, Kil Yong Kim, Jang Sun Suh, Yong Woong Kim, Ro-Dong Park, Hoon Hwangbo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Enterobacter
Biology Poaceae Gluconates Plant Roots Applied Microbiology and Biotechnology Microbiology High-performance liquid chromatography Phosphates chemistry.chemical_compound 2-ketogluconic acid Soil Microbiology Enterobacter intermedium Rhizosphere Chromatography General Medicine Hydrogen-Ion Concentration Phosphate biology.organism_classification Culture Media Solubility chemistry Biochemistry Solubilization Gluconic acid Bacteria |
| Zdroj: | Current Microbiology. 47:87-92 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-002-3951-y |
| Popis: | Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200–250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period. RID=”” ID=”” Correspondence to: K.Y. Kim; email: kimkilcchonnam.ac.kr |
| Databáze: | OpenAIRE |
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