Molecular chracterisation of porcine group A rotaviruses: Studies on the age-related occurrence and spatial distribution of circulating virus genotypes in Poland
| Autor: | Jerzy Kozyra, A. Dors, Iwona Kozyra, Artur Rzeżutka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diarrhea
Rotavirus Veterinary medicine Genotype Swine Population Genome Viral Biology medicine.disease_cause Microbiology Rotavirus Infections Virus Feces 03 medical and health sciences Prevalence medicine Animals Pig farming Genetic variability education Phylogeny 030304 developmental biology Swine Diseases 0303 health sciences education.field_of_study General Veterinary 030306 microbiology Host (biology) Age Factors Genetic Variation Sequence Analysis DNA General Medicine Herd Poland |
| Zdroj: | Veterinary Microbiology. 232:105-113 |
| ISSN: | 0378-1135 |
| Popis: | Rotaviruses of group A (RVAs) commonly occur in farm animals. In pigs, they cause acute gastrointestinal disease which is considered as significant factor of economic losses in pig farming. The aim of the study was an assessment of the prevalence of rotavirus (RV) infections in farmed pigs in Poland, genotype identification of the virus strains in conjunction with their age-related occurrence and regional (province) distribution pattern in pig herds. In total, 920 pig faecal samples were collected from pigs between the ages of one week and two years old from 131 farms. RVAs were detected using ELISA and molecular methods followed by a sequence-based identification of G (VP7) and P (VP4) virus genotypes. RV antigen was found in 377 (41%) of pig faecal samples. The correlation between pig age and frequency of RV infections was shown. In the Polish pig population, 145 RVA strains representing 33 GP genotypes were identified. Subsequent molecular analysis revealed an age-dependent and regional diversity in distribution of genotypes and virus strains. Besides typical pig RVA strains, novel strains such as G5P [34], G9P[34], and human G1P[8] were identified in this animal host. Findings from this study showed a change over time in the genotype occurrence of circulating pig RVAs in Poland. The high genetic variability of RV strains and acquisition of new virus genotypes have led to the emergence of novel, genetically distinct RVAs. The changes in the genotype occurrence of RVA strains in pigs indicate the need for their continuous epidemiological surveillance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect