Guanosine triphosphate analysis: PEI-thin layer purification and luciferin-luciferase liquid scintillation quantitation
| Autor: | Knox Van Dyke, Madhu S.P. Manandhar |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
GTP'
Biophysics Firefly Luciferin Guanosine triphosphate Cytoplasmic Granules Tritium Biochemistry chemistry.chemical_compound Adenosine Triphosphate Methods medicine Animals Nucleotide Luciferase Luciferases Molecular Biology chemistry.chemical_classification Chromatography Adenine Nucleotides Microchemistry Liquid scintillation counting Cell Biology Luciferin Adenosine Adenosine Monophosphate chemistry Adrenal Medulla Scintillation Counting Cattle Light emission Chromatography Thin Layer Guanosine Triphosphate medicine.drug |
| Zdroj: | Analytical Biochemistry. 60:122-129 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(74)90136-5 |
| Popis: | A novel method to purify and quantitate GTP is described. This method is simple to run and sensitive to detect GTP in picomole quantities. It is based on purfication and isolation of GTP on PEI-thin layer chromatograms and quantitation with the firefly luciferin-luciferase system. GTP characteristically gives a delayed (about 2 min) peak light emission similar to adenosine tetraphosphate (about 6 min) but different from ATP which gives a peak light emission value at zero time. The light emission from each of these three nucleotides is characteristically different from one another such that contamination between these three nucleotides can be detected by their peak light emission times. This method has been applied to analyze GTP in biological preparations which appeared to be free of detectable concentration(s) of interfering substances(s). |
| Databáze: | OpenAIRE |
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