Effect of CTLA4‐Ig (abatacept) treatment on T cells and B cells in peripheral blood of patients with polymyositis and dermatomyositis
Autor: | Olga Krystufkova, Ingrid E. Lundberg, Yang Chen, Adnane Achour, Jiri Vencovsky, Vivianne Malmström, Cecilia Wick, Herman Mann, Quan Tang, Petter Brodin, Helene Alexanderson, Maryam Dastmalchi, Jaromír Mikeš, Daniel Ramsköld, Tadepally Lakshmikanth |
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Rok vydání: | 2018 |
Předmět: |
Adult
Male 0301 basic medicine T cell Immunology B-Lymphocyte Subsets Polymyositis Peripheral blood mononuclear cell Dermatomyositis Abatacept 03 medical and health sciences T-Lymphocyte Subsets Humans Medicine Mass cytometry Myositis business.industry General Medicine Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure Female business Immunosuppressive Agents CD8 medicine.drug |
Zdroj: | Scandinavian Journal of Immunology. :e12732 |
ISSN: | 1365-3083 0300-9475 |
Popis: | We aimed to evaluate in vivo effects of abatacept on phenotypes of T and B cells in the circulation of myositis patients in a sub-study of the ARTEMIS trial. Twelve patients with paired frozen PBMCs before and after 6-month abatacept treatment were included in this sub-study where mass cytometry (CyTOF) was chosen as a technology to be tested for its utility in a real-life clinical immune monitoring setting. Using CyTOF, the peripheral T cell phenotypes demonstrated considerable variation over time and between individuals precluding the identification of treatment-specific changes. We therefore conclude that studies of patient cohorts displaying wide clinical heterogeneity using mass cytometry must be relatively large in order to be suited for discovery research and immune monitoring. Still, we did find some correlations with functional muscle outcome, namely positive correlations between the ratio of CD4+ T cells and CD8+ T cells (CD4/CD8) in peripheral blood samples both at baseline and after treatment with muscle endurance improvement as assessed by the functional index-2 (FI-2) test. Our data suggest that the CD4/CD8 ratio in circulation at time of active disease may be a predictor of treatment efficacy in myositis patients. |
Databáze: | OpenAIRE |
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