The Axial Element Protein DESYNAPTIC2 Mediates Meiotic Double-Strand Break Formation and Synaptonemal Complex Assembly in Maize
| Autor: | Chung-Ju Rachel Wang, Robert B. Meeley, Ding Hua Lee, Jia-Chi Ku, Ya-Shiun Jan, Chien-Yu Lin, Yu-Hsin Kao |
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| Rok vydání: | 2015 |
| Předmět: |
Synaptonemal Complex
Molecular Sequence Data Synapsis RAD51 Cell Biology Plant Science Biology Zea mays Molecular biology Synaptonemal complex assembly Chromosomal crossover Cell biology Meiosis Synaptonemal complex Gene Expression Regulation Plant Mutation Homologous chromosome DNA Breaks Double-Stranded Homologous Recombination Central element Research Articles Plant Proteins |
| Zdroj: | The Plant Cell. 27:2516-2529 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.15.00434 |
| Popis: | During meiosis, homologous chromosomes pair and recombine via repair of programmed DNA double-strand breaks (DSBs). DSBs are formed in the context of chromatin loops, which are anchored to the proteinaceous axial element (AE). The AE later serves as a framework to assemble the synaptonemal complex (SC) that provides a transient but tight connection between homologous chromosomes. Here, we showed that DESYNAPTIC2 (DSY2), a coiled-coil protein, mediates DSB formation and is directly involved in SC assembly in maize (Zea mays). The dsy2 mutant exhibits homologous pairing defects, leading to sterility. Analyses revealed that DSB formation and the number of RADIATION SENSITIVE51 (RAD51) foci are largely reduced, and synapsis is completely abolished in dsy2 meiocytes. Super-resolution structured illumination microscopy showed that DSY2 is located on the AE and forms a distinct alternating pattern with the HORMA-domain protein ASYNAPTIC1 (ASY1). In the dsy2 mutant, localization of ASY1 is affected, and loading of the central element ZIPPER1 (ZYP1) is disrupted. Yeast two-hybrid and bimolecular fluorescence complementation experiments further demonstrated that ZYP1 interacts with DSY2 but does not interact with ASY1. Therefore, DSY2, an AE protein, not only mediates DSB formation but also bridges the AE and central element of SC during meiosis. |
| Databáze: | OpenAIRE |
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