An autonomous CMOS hysteretic sensor for the detection of desorption-free DNA hybridization
| Autor: | Jeong Oen Lee, Kang-Ho Lee, Jun-Bo Yoon, Sukhwan Choi, Gyu-Hyeong Cho, Mi-Jin Sohn |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Capacitive sensing Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques Integrated circuit Electric Capacitance Capacitance law.invention Hardware_GENERAL law Electric Impedance Hardware_INTEGRATEDCIRCUITS Electrochemistry Electrical impedance Base Sequence Influenza A Virus H5N1 Subtype business.industry Detector Nucleic Acid Hybridization Electrochemical Techniques General Medicine Chip CMOS Dielectric Spectroscopy DNA Viral Optoelectronics DNA Probes business Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 26:4591-4595 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2011.04.033 |
| Popis: | This paper describes a sensor for label-free, fully electrical detection of DNA hybridization based on capacitive changes in the electrode-electrolyte interface. The sensor measures capacitive changes in real time according to a charging-discharging principle that is limited by the hysteresis window. In addition, a novel autonomous searching technique, which exclusively monitors desorption-free hybridized electrodes among electrode arrays, enhances the performance of the sensor compared with conventional capacitive measurement. The sensor system achieves a detection range of 80 dB. The integrated circuit sensor is fabricated with a 0.35 μm CMOS process. The proposed sensor offers rapid, robust and inexpensive measurement of capacitance with highly integrated detection circuitry. It also facilitates quantitative evaluations of molecular densities on a chip with distinctive impedance variations by monitoring desorption-free hybridized electrodes. Our electrical biosensor has great potential for use with bio analytical tools and point-of-care diagnosis. |
| Databáze: | OpenAIRE |
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