Enantioselective multidimensional gas chromatography–mass spectrometry in the analysis of urinary organic acids
| Autor: | Thomas Beck, Martin Heil, Frank Podebrad, Adrian C. Sewell, Hans Böhles, Armin Mosandl |
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| Rok vydání: | 1998 |
| Předmět: |
Male
chemistry.chemical_classification Chromatography Carboxylic Acids Enantioselective synthesis Infant Stereoisomerism General Chemistry Mass spectrometry Gas Chromatography-Mass Spectrometry Pyrrolidonecarboxylic Acid Amino acid Lactic acid chemistry.chemical_compound chemistry Child Preschool Humans Female Pyroglutamic acid Enantiomer Gas chromatography–mass spectrometry Child Metabolism Inborn Errors Organic acid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 714:119-126 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(98)00233-3 |
| Popis: | Enantioselective multidimensional gas chromatography-mass spectrometry is a valuable tool for the enantioselective analysis of compounds from complex matrices. Samples are separated initially on a precolumn and selected substances then transferred on-line to a main-column coated with suitable chiral stationary phase for enantioselective analysis with subsequent mass selective detection. In this paper the method is used as an adjunct to urinary organic acid analysis to provide information in patients with suspected inborn errors of metabolism. Lactic acid, alpha-hydroxyisocaproic acid, 3-phenyllactic acid and 3-(4-hydroxyphenyl)-lactic acid are separated in a single run. In addition, the enantioselective analysis of pyroglutamic acid is presented. D-Enantiomers of amino acids and alpha-hydroxycarboxylic acids derived from amino acids, point to a bacterial origin whereas the L-enantiomer is predominantly of endogenous origin. Therefore the enantiomeric ratio of chiral metabolites is an important parameter for the understanding of metabolic processes. |
| Databáze: | OpenAIRE |
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