Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets
| Autor: | Andrew J. Quantock, Yoshinori Oie, Tomoyo Tanaka, Takahiro Ogasawara, Masukazu Inoie, Kenichiro Hata, Kohji Nishida, Mio Morita, Yosuke Teranishi, Kei Sasaki, Koichi Baba, Izumi Kusumi, Masahiro Kino-oka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Corneal epithelial cell Corneal diseases Cell Biomedical Engineering lcsh:Medicine Limbus Corneae Biology Article 3T3 cells Cell Line Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Limbal stem cell lcsh:Science Cell Proliferation Multidisciplinary Stem Cells lcsh:R Epithelium Corneal Membrane Proteins Cell Differentiation Epithelial Cells 3T3 Cells Middle Aged Epithelium eye diseases Cell biology Transplantation 030104 developmental biology medicine.anatomical_structure Cell culture 030221 ophthalmology & optometry Female lcsh:Q Thickening sense organs Stem Cell Transplantation |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-64394-6 |
| Popis: | Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it’s presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets. |
| Databáze: | OpenAIRE |
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