Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland
| Autor: | Paul M. Bartley, H Stevenson, Helen Carty, Francesca Chianini, S Guido, E.A. Innes, Frank Katzer, CS Mason |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Veterinary medicine Farms 040301 veterinary sciences Placenta Cattle Diseases Sheep Diseases Abortion 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Neospora Pregnancy parasitic diseases medicine Parasite hosting Animals Fetus Sheep biology Brain Heart 04 agricultural and veterinary sciences 030108 mycology & parasitology Abortion Veterinary DNA Protozoan biology.organism_classification Neospora caninum Infectious Diseases medicine.anatomical_structure chemistry Pregnancy Complications Parasitic Aborted Fetus Animal Science and Zoology Parasitology Cattle DNA Intergenic Female Flock DNA |
| Zdroj: | Parasitology. 146(7) |
| ISSN: | 1469-8161 |
| Popis: | Neospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6% (14.2–21.4)], 7/25 heart [28.0% (12.1–49.4)] and 5/21 placenta [23.8% (8.2–47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0% (14.6–21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks. |
| Databáze: | OpenAIRE |
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