Artificial biomembrane based on DPPC — Investigation into phase transition and thermal behavior through ellipsometric techniques
Autor: | Felipe Droguett, Guadalupe Pizarro-Guerra, Mauricio Sarabia, Carmen González |
---|---|
Jazyk: | angličtina |
Předmět: |
Surface wettability
Materials science 1 2-Dipalmitoylphosphatidylcholine Scanning electron microscope Thin films Lipid Bilayers Analytical chemistry Biophysics Ellipsometric measurements Micelle Biochemistry Phase Transition Contact angle symbols.namesake Biomimetic Materials Materials Testing Ripple phase Wafer Thin film Spectrum Analysis Bilayer Temperature technology industry and agriculture Cell Biology Refractometry Chemical engineering symbols lipids (amino acids peptides and proteins) Wetting Raman spectroscopy |
Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. (10):2295-2307 |
ISSN: | 0005-2736 |
DOI: | 10.1016/j.bbamem.2015.07.001 |
Popis: | Organic thin film deposition presents a multiplicity of challenges. Most notably, layer thickness control, homogeneity and subsequent characterization have been not cleared yet. Phospholipid bilayers are frequently used to model cell membranes. Bilayers can be disrupted by changes in mechanical stress, pH and temperature. The strategy presented in this article is based on thermal study of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) through analysis of slight changes in material thickness. The sample was prepared by depositing X- or Y-type DPPC bilayers using Langmuir–Blodgett technique over silicon wafer. Thus, molecular inclination degree, mobility and stability of phases and their respective phase transitions were observed and analyzed through ellipsometric techniques during heating cycles and corroborated by Grazing Incidence X-ray Diffraction and Atomic Force Microcopy measurements. DPPC functional group vibrations were detected by Raman spectra analysis. Scanning Electron Microscope with Field Emission gun (FE-SEM) and conventional SEM micrographs were also used to characterize sample morphology, demonstrating that homogenous bilayer formations coexist with some vesicles or micelles at surface level. Contact angle measurements corroborate DPPC surface wettability, which is mainly related to surface treatment methods of silicon wafer used to create either hydrophilic or hydrophobic nature regarding the substrate surface. Also, shifting and intensity changes of certain functional groups into Raman spectra confirm water presence between DPPC layers. Signal analysis detects certain interdigitation between aliphatic chains. These studies correspond to the base of future biosensors based on proteins or antimicrobial peptides stabilized into phospholipid bilayers over thin hydrogel films as moist scaffold. |
Databáze: | OpenAIRE |
Externí odkaz: |