An evaluation of lipid metabolism in the insect trypanosomatid Herpetomonas muscarum uncovers a pathway for the uptake of extracellular insect lipoproteins
| Autor: | Lívia Silva-Cardoso, George Eduardo Gabriel Kluck, Narcisa L. Cunha-Silva, Patrícia Fampa, Alexandre Romeiro, Karla C. Régis, Miria G. Pereira, Georgia C. Atella, Alessandra C. Chagas-Lima, Nuccia N.T. De Cicco |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatography Gas Insecta Lipoproteins 030231 tropical medicine Euglenozoa Infections Biology Mass Spectrometry Host-Parasite Interactions Palmitic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hemolymph Extracellular Animals Parasite hosting Binding site Fatty Acids Lipid metabolism Metabolism Lipid Metabolism Biosynthetic Pathways Sterols Metabolic pathway 030104 developmental biology Infectious Diseases chemistry Biochemistry Trypanosomatina Parasitology |
| Zdroj: | Parasitology International. 67:97-106 |
| ISSN: | 1383-5769 |
| Popis: | Lipid uptake and metabolism by trypanosomatid parasites from vertebrate host blood have been well established in the literature. However, there is a lack of knowledge regarding the same aspects concerning the parasites that cross the hemolymph of their invertebrate hosts. We have investigated the lipid composition and metabolism of the insect trypanosomatid Herpetomonas muscarum by 3H- palmitic acid and phosphate (32Pi) and the parasite interaction with Lipophorin (Lp) the main lipid carrying protein of insect hemolymph. Gas chromatography-mass spectrometry (GC-MS) analyses were used to identify the fatty acids and sterols composition of H.muscarum. Furthermore, we investigated the Lp binding site in the plasma membrane of parasite by Immunolocalization. We showed that H. muscarum incorporated 3H-palmitic acid and inorganic phosphate (32Pi) which were readily used as precursor molecules of lipid biosynthetic pathways. Furthermore, H. muscarum was able to take up both protein and lipid moieties of Lp which could be used as nutrient sources. Moreover, we have also demonstrated for the first time the presence of a Lp binding site in the membrane of a parasite. Such results point out the role of describing the metabolic pathways of trypanosomatids in order to provide a better understanding of parasite-host interaction peculiarities. Such studies may enhance the potential form the identification of novel chemotherapeutic targets in harmful parasites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect