Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia
| Autor: | Fabián Cortés-Mancera, María Cristina Navas, Julio César Rendón, Juan Carlos Restrepo-Gutiérrez, Sergio Hoyos |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
RNA viruses 0301 basic medicine medicine.medical_treatment lcsh:Medicine Hepacivirus Liver transplantation medicine.disease_cause Liver disease Risk Factors Genotype Medicine and Health Sciences Medicine ORFS lcsh:Science Pathology and laboratory medicine Multidisciplinary Hepatitis C virus Liver Diseases Database and informatics methods Microbial Mutation Sequence analysis Middle Aged Medical microbiology Viral Load Hepatitis B Cirrhosis Viruses Female Pathogens Viral load Research Article Adult Hepatitis B virus Bioinformatics Surgical and Invasive Medical Procedures Gastroenterology and Hepatology Genome Viral Colombia Microbiology End Stage Liver Disease Liver inflammation Digestive System Procedures 03 medical and health sciences Hepatitis B Chronic Genetics Humans Point Mutation DNA sequence analysis Liver diseases Transplantation Hepatitis B Surface Antigens Flaviviruses business.industry lcsh:R Organisms Viral pathogens Biology and Life Sciences Organ Transplantation medicine.disease Virology Hepatitis viruses Microbial pathogens Liver Transplantation Research and analysis methods 030104 developmental biology Mutation lcsh:Q business Nested polymerase chain reaction |
| Zdroj: | PLoS ONE, Vol 12, Iss 7, p e0180447 (2017) Repositorio UdeA Universidad de Antioquia instacron:Universidad de Antioquia PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Hepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease. Methods Sixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique. Results In five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X. Conclusions This is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis. |
| Databáze: | OpenAIRE |
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