Isolation and analysis of peptidic fragments of alpha-gliadin using reversed-phase high-performance liquid chromatography
| Autor: | W.Th.J.M. Hekkens, Marie Kočová-Holáková, Slabý J, Evžen Kasafírek, Petr Kocna, Přemysl Frič |
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| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
Chromatography Molecular mass Electrophoresis Starch Gel Ultrafiltration Fraction (chemistry) Peptide General Chemistry Fractionation High-performance liquid chromatography Gliadin Peptide Fragments chemistry.chemical_compound chemistry Trifluoroacetic acid Acetonitrile Chromatography High Pressure Liquid Plant Proteins |
| Zdroj: | Scopus-Elsevier |
| Popis: | Summary Peptidic fragments of α-gliadin were obtained by peptic-tryptic-pancreatic (PTP) digestion of the α-gliadin fraction isolated by ion-exchange chromatography on a sulphopropyl-Sephadex C-50 column. The proteolytic digest was fractionated by ultrafiltration into three subfractions, PTPa 1 –PTPa 3 . The subfraction PTPa 2 was then analysed and individual peaks were separated using reversed-phase high-performance liquid chromatography (RP-HPLC) using a gradient of acetonitrile in 0.1% trifluoroacetic acid and a Separon SGX-C 18 sorbent. A 100-mg amount of the PTPa 2 subfraction was separated in a single analysis by preparative RP-HPLC and twenty peaks were obtained for further characterization. The molecular mass in range 300–3000 was established for individual peptidic fragments by gel-permeation chromatography on a TSK-G2000 SW column. |
| Databáze: | OpenAIRE |
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