Notch 2 signaling contributes to cell growth, anti-apoptosis and metastasis in laryngeal squamous cell carcinoma
| Autor: | Fang Fang, Chen Chen, Shi‑Ming Chen, Yong Jun Ding, You Zou, Xing Yi, Meng‑Yuan Dai, Ze‑Zhang Tao |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty proliferation Notch signaling pathway Biology Biochemistry Metastasis 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Genetics medicine Humans metastasis Neoplasm Invasiveness Receptor Notch2 Neoplasm Metastasis Notch 2 Molecular Biology Laryngeal Neoplasms Cell Proliferation Oncogene Cell growth apoptosis Articles Cell cycle medicine.disease Lymphoma Gene Expression Regulation Neoplastic 030104 developmental biology laryngeal squamous cell carcinoma Oncology 030220 oncology & carcinogenesis Cancer cell Carcinoma Squamous Cell Molecular Medicine RNA Interference Larynx Signal Transduction |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Notch signaling is important during the development of a variety of human tumors. Depending on the context, Notch signaling can be either oncogenic or anti-proliferative, and therefore, its effects in cancer are unpredictable. The aim of the present study was to identify the importance of Notch 2 in the cell growth and metastasis of laryngeal squamous cell carcinoma (LSCC). The current study performed quantum dots-based immunofluorescence histochemistry to determine expression of Notch 2 in 72 LSCC samples without lymph node metastasis, 23 LSCC samples with lymph node metastasis and 31 samples from vocal cord polyps. It was observed that Notch 2 was upregulated in LSCC tissue compared with normal vocal cord polyps. This upregulation was further enhanced in LSCC tissues with lymph node metastasis compared with LSCC tissues without lymph node metastasis. Following knockdown of NOTCH2 expression in LSCC cells, the in vitro tumorigenicity of Hep-2 cells was inhibited, with growth, migration, invasion and proliferation reduced, and apoptosis induced. Additionally, following downregulation of Notch 2 protein expression, the protein expression levels of phosphor-mitogen-activated protein kinase 1 (p-ERK), v-myc avian myelocytomatosis viral oncogene homolog and B-cell CLL/lymphoma 2 (Bcl2) were also downregulated, whereas, Bcl2-associated X protein expression was upregulated. There were no changes detected in the protein expression levels of total-ERK, phospho-v-akt murine thymoma viral oncogene homolog 1 (p-Akt) and total-Akt. The results of the present study suggest that Notch 2 is important for the cell growth, anti-apoptosis and metastasis of LSCC. Therefore, Notch 2 inhibitors may have therapeutic potential for the treatment of patients with LSCC via the inhibition of cancer cell growth and metastasis. |
| Databáze: | OpenAIRE |
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