Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials
| Autor: | M. Alejandra Sanchez, Luciano D. Sappia, Silio Lima Moura, M. Isabel Pividori, Betiana Felice, Mercè Martí |
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| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Materials science Bone Regeneration Cell Culture Techniques Bioengineering 02 engineering and technology Calorimetry 010402 general chemistry Exosomes 01 natural sciences Nanomaterials Cell Line Biomaterials Non destructive medicine Humans Bone Development Osteoblasts Biomaterial Osteoblast musculoskeletal system 021001 nanoscience & nanotechnology Alkaline Phosphatase Microvesicles 0104 chemical sciences Cell biology Culture Media medicine.anatomical_structure Mechanics of Materials Alkaline phosphatase Cell culture supernatant Stem cell 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 115 |
| ISSN: | 1873-0191 |
| Popis: | Alkaline phosphatase (ALP) is an essential biomarker of osteoblastic activity. Currently, ALP activity has been used to study bone mineralization mechanisms and osteoactive biomaterials among others. The ALP quantification is usually performed by destructive methods either on growing cells or cells lysate in which the osteoconductive biomaterial is being assessed. This work addresses the evaluation of a non-destructive colorimetric approach for the determination of ALP activity on osteoblast-derived exosomes from culture supernatants. The efficiency of the method was evaluated on osteoconductive electrospun scaffolds of PCL compounded with ZnO as a reference biomaterial. The results demonstrated that the osteoblast cell line mineralization induced by osteoconductive scaffolds can be monitorized over time by the non-destructive measurement of ALP activity on osteoblast derived exosomes. Consequently, this non-destructive approach suggested to be a reliable alternative technique for the quantification of biomaterials osteoconductivity or even evaluation of osteoblastic response at stem cells. |
| Databáze: | OpenAIRE |
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