Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166

Autor: Pierre van der Bruggen, Camille Lemaigre, Cesar Augusto Valades-Cruz, Henri-François Renard, François Tyckaert, Ludger Johannes, Ruddy Wattiez, Cristina Lo Giudice, Pierre Morsomme, David Alsteens, Massiullah Shafaq-Zadah, Christian Wunder, Thibault Hirsch
Přispěvatelé: UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology, Bodescot, Myriam, Assimilation de Données et Microscopie à Feuille de Lumière Structurée pour la Modélisation des Voies d'Endocytose et d'Exocytose en Cellule Unique - - DALLISH2016 - ANR-16-CE23-0005 - AAPG2016 - VALID, Développment d'une infrastructure française distribuée coordonnée - - France-BioImaging2010 - ANR-10-INBS-0004 - INBS - VALID, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, Endocytic Membrane Compartmentalization by Galectins - GALECTCOMPART - - EC:FP7:ERC2014-04-01 - 2019-03-31 - 340485 - VALID, Université Catholique de Louvain = Catholic University of Louvain (UCL), Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes (SERPICO), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Mons (UMons), Walloon Excellence in Life sciences and BIOtechnology [Liège] (WELBIO), This work was supported by grants from the 'Fonds National de la Recherche Scientifique' (FNRS, CDR-J.0119.19) and the 'Communauté française de Belgique–Actions de Recherches Concertées' (17/22-085). This work was also supported by the French National Research Agency (DALLISH–ANR-16-CE23-0005), and by Inria in the frame of NAVISCOPE-IPL (Inria Project Lab). The bioprofiling platform used for the proteomic analysis was supported by the FNRS, the European Regional Development Fund, and the Walloon Region, Belgium. The LLSM was financed by PIA France-Bioimaging (ANR-10-INBS-04_01), LabEx DCBiol, LabEx CelTisPhyBio ANR-11-LABX-0038, Agence Nationale de la Recherche (ANR-16-CE23-0005-02, ANR-19-CE13-0001-01), HFSP (RGP0029/2014), and European Research Council (ERC project 340485). We greatly acknowledge the Cell and Tissue Imaging Facility (PICT-IBiSA) and Nikon Imaging Centre, Institut Curie, member of the French National Research Infrastructure France-BioImaging (ANR-10-INBS-04). H.-F.R. is a FNRS postdoctoral research fellow (Belgium). F.T., T.H. and C.L. are supported by PhD fellowships from FRIA/FNRS (Belgium). C.L.G. is an EMBO Long-term postdoctoral fellow. P.V.D.B. and D.A. are supported by WELBIO (Fédération Wallonie-Bruxelles, Belgium). This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 758224). D.A. is a research associate of the FNRS (Belgium)., ANR-16-CE23-0005,DALLISH,Assimilation de Données et Microscopie à Feuille de Lumière Structurée pour la Modélisation des Voies d'Endocytose et d'Exocytose en Cellule Unique(2016), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), European Project: 340485,EC:FP7:ERC,ERC-2013-ADG,GALECTCOMPART(2014), UCL - SSS/DDUV - Institut de Duve, Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)
Rok vydání: 2020
Předmět:
Fetal Proteins
0301 basic medicine
Intravital Microscopy
Endocytic cycle
General Physics and Astronomy
Mice
0302 clinical medicine
Cell Movement
Neoplasms
Chlorocebus aethiops
RNA
Small Interfering
lcsh:Science
Multidisciplinary
Cell adhesion molecule
Chemistry
Activated-Leukocyte Cell Adhesion Molecule
Signal transducing adaptor protein
Endocytosis
Recombinant Proteins
3. Good health
Cell biology
Galectin-8
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Science
Cell Adhesion Molecules
Neuronal
Galectins
[SDV.CAN]Life Sciences [q-bio]/Cancer
Genetics and Molecular Biology
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
[SDV.CAN] Life Sciences [q-bio]/Cancer
Antigens
CD
Cell Line
Tumor
Cell Adhesion
Animals
Humans
Cell adhesion
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
ALCAM
Adaptor Proteins
Signal Transducing
Cell Membrane
General Chemistry
Fibroblasts
Clathrin
030104 developmental biology
General Biochemistry
lcsh:Q
Zdroj: Nature Communications, Vol. 11, no.1, p. 1457 (2020)
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Nature Communications, 2020, 11 (1), pp.1457. ⟨10.1038/s41467-020-15303-y⟩
Nature Communications, Vol. 11, no. 1457, p. 1-13 (2020)
Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.1457. ⟨10.1038/s41467-020-15303-y⟩
ISSN: 2041-1723
DOI: 10.1038/s41467-020-15303-y
Popis: While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a clathrin-independent cargo. We show that endophilin-A3—but neither A1 nor A2 isoforms—functionally associates with CD166-containing early endocytic carriers and physically interacts with the cargo. Our data further demonstrates that the three endophilin-A isoforms control the uptake of distinct subsets of cargoes. In addition, we provide strong evidence that the construction of endocytic sites from which CD166 is taken up in an endophilin-A3-dependent manner is driven by extracellular galectin-8. Taken together, our data reveal the existence of a previously uncharacterized clathrin-independent endocytic modality, that modulates the abundance of CD166 at the cell surface, and regulates adhesive and migratory properties of cancer cells.
How and which cell surface molecules are taken up by clathrin-independent endocytosis is an ongoing area of research. Here, the authors show that the tumor marker CD166 is a clathrin-independent cargo that is taken up by endophilin-A3 and galectin-8, which regulates cancer cell migration.
Databáze: OpenAIRE
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