Spinal cord injury and its underlying mechanism in rats with temporal lobe epilepsy
Autor: | Jinjie Liu, Kai Gao, Guoliang Liu, Sibo Liu, Yongbo Zhao, Zhou Hengjie, Lin Zhang, Hong Wang, Zanhua Liu |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty seizure Inflammation 03 medical and health sciences Epilepsy symbols.namesake chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) medicine Spinal cord injury brain blood barrier albumin Evans Blue business.industry General Medicine Articles temporal lobe epilepsy medicine.disease Spinal cord Extravasation spinal cord injury Staining 030104 developmental biology medicine.anatomical_structure chemistry nervous system inflammation 030220 oncology & carcinogenesis Nissl body symbols medicine.symptom business |
Zdroj: | Experimental and Therapeutic Medicine |
ISSN: | 1792-1015 1792-0981 |
Popis: | Numerous cases of spinal cord injury following seizure have been previously reported. However, whether spinal cord injury is a common occurrence after seizures and its underlying mechanisms remain unclear. The present study generated a Sprague-Dawley rat model of temporal lobe epilepsy (TLE), and Nissl staining and transmission electron microscopy were used to detect tissue damage. In addition, Evans blue staining was used to detect damage to the blood-brain barrier (BBB) and albumin extravasation. In addition, double-staining was used to detect the association between neurons and extravasated albumin. Furthermore, neuronal degeneration was assessed using Fluoro-Jade C staining, while fluorescence staining and western blotting were used to detect apoptosis and inflammation. In the present study, spinal cord injury was only observed in rats with grade IV-V seizures, whereas Nissl staining showed structural damage and decreased neuronal cell numbers in the brain and the spinal cord. The present study identified BBB damage and albumin extravasation in rats of the TLE groups. Double-staining for albumin and neurons showed a significant match of neurons positive for albumin. Fluoro-Jade C staining indicated neuronal degeneration in the brain, but not the spinal cord in the TLE rats. High levels of caspase-3 were also detected in the injured spinal cord. A small number of albumin+ neurons in the spinal cord presented caspase-3+ signals in rats of the TLE groups. The expression levels of intercellular adhesion molecule 1, CD11b and inflammatory factors such as tumor necrosis factor-α and interleukin-6 were significantly elevated in the injured spinal cord. The present results suggested that spinal cord injury occurred in rats as a result of severe seizure attacks, and that BBB damage, albumin extravasation, inflammation and apoptosis contributed to the pathological changes observed during spinal cord injury. |
Databáze: | OpenAIRE |
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