Heterogeneous inducible mammary-specific expression of JAB/SOCS1 in lactating transgenic mice is associated with no obvious phenotype, even at the cellular level
| Autor: | Solange Soulier, M. Hudrisier, Jean Djiane, Barbara Petridou, José Costa Da Silva, Catherine Hue-Beauvais, Nathalie Besnard, Jean-Luc Vilotte |
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| Přispěvatelé: | Unité de recherche génomique et physiologie de la lactation (GPL), Institut National de la Recherche Agronomique (INRA), Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Mammary gland Suppressor of Cytokine Signaling Proteins Mice Pregnancy Lactation Cricetinae Gene expression Transgenes Promoter Regions Genetic STAT5 ComputingMilieux_MISCELLANEOUS TRANSGENIC MICE 0303 health sciences biology 030302 biochemistry & molecular biology MAMMARY GLAND Milk Proteins Immunohistochemistry GENOMIQUE 3. Good health Cell biology medicine.anatomical_structure Phenotype Female Biotechnology Genetically modified mouse medicine.medical_specialty Transgene Mice Transgenic CHO Cells Suppressor of cytokine signalling 03 medical and health sciences Cricetulus Mammary Glands Animal Suppressor of Cytokine Signaling 1 Protein Internal medicine Genetics medicine Animals [INFO]Computer Science [cs] 030304 developmental biology Suppressor of cytokine signaling 1 SUPPRESSOR OF CYTOKINE SIGNALLING 1 Repressor Proteins Endocrinology biology.protein Animal Science and Zoology Carrier Proteins Agronomy and Crop Science CONDITIONNAL EXPRESSION |
| Zdroj: | Transgenic Research Transgenic Research, Springer Verlag, 2003, 12, pp.693-706 FEBS (Federation of European Biochemical Societies) Meeting on Signal Transduction FEBS (Federation of European Biochemical Societies) Meeting on Signal Transduction, Jul 2003, Bruxelles, Belgium. 1 p., 2003 |
| ISSN: | 0962-8819 1573-9368 |
| Popis: | The cytokine-inducible suppressor of cytokine signalling SOCS1, or JAB, has been shown to be implicated in vitro in the negative regulation of the prolactin-receptor-induced activation of JAK2 and STAT5. Disruption of this gene in vivo resulted in an accelerated mammary gland development. In the present experiment, we assessed the potential impact on the lactation process of the doxycycline-inducible mammary-controlled expression of this gene in transgenic mice. Three transgenic mouse lines that expressed JAB specifically in the mammary gland in a conditional manner following doxycycline treatment were successfully established. The resulting overall expression of JAB was high and ranged from half to four times that of the endogenously expressed homologous gene in the thymus. It was found to be highly heterogeneous in the mammary epithelium, with less than 5% of JAB-expressing cells detected. Phenotypic analysis of these transgenic mice exhibiting doxycycline-induced JAB expression did not reveal any obvious effect on the lactation process. Double immunostaining experiments suggested that JAB expression in vivo did not significantly affect the beta-casein gene expression and the STAT5a nuclear localisation. These results do not support a role for JAB in the disruption of the lactation process. |
| Databáze: | OpenAIRE |
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