Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis
| Autor: | Rui Wang, Jing-Xin Mao, Xiao-Long Zhang, Xiaozhong Lan, Min Chen, Fan-Cheng Meng, Zhaoyue Dong, Zhihua Liao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Necrosis Cell Survival Health Toxicology and Mutagenesis Pterocephalus hookeri lcsh:Medicine Pharmacology n-butanol extract Toxicology Carrageenan Caprifoliaceae Article Cell Line necrosis 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Western blot In vivo Lactate dehydrogenase medicine Animals Edema Humans Propidium iodide Protein kinase A 030304 developmental biology 0303 health sciences medicine.diagnostic_test Plant Extracts Cell Membrane GTPase-Activating Proteins lcsh:R NF-kappa B liver toxicity In vitro chemistry Liver inflammation 030220 oncology & carcinogenesis Receptor-Interacting Protein Serine-Threonine Kinases Toxicity Female medicine.symptom Chemical and Drug Induced Liver Injury |
| Zdroj: | Toxins, Vol 11, Iss 3, p 142 (2019) Toxins Volume 11 Issue 3 |
| ISSN: | 2072-6651 |
| Popis: | Pterocephalus hookeri (C. B. Clarke) Hö eck, recorded in the Chinese Pharmacopoeia (2015 version) as a Tibetan medicine for the treatment of various diseases, especially rheumatoid arthritis, was believed to possess a slight toxicity. However, hardly any research has been carried out about it. The present study aimed to evaluate the toxicity in vivo and in vitro. Toxicity was observed by the evaluation of mice weight loss and histopathological changes in the liver. Then, the comparison research between ethyl acetate extract (EAE) and n-butanol extract (BUE) suggested that liver toxicity was mainly induced by BUE. The mechanical study suggested that BUE-induced liver toxicity was closely associated with necrosis detected by MTT and propidium iodide (PI) staining, via releasing lactate dehydrogenase (LDH), reducing the fluidity, and increasing the permeability of the cell membrane. Western blot analysis confirmed that the necrosis occurred molecularly by the up-regulation of receptor-interacting protein kinase 1 (RIP1) and receptor-interacting protein kinase 3 (RIP3), as well as the activation of the nuclear factor-kappa-gene binding (NF-&kappa B) signaling pathway in vivo and in vitro. This finding indicated that the liver toxicity induced by BUE from P. hookeri was mainly caused by necrosis, which provides an important theoretical support for further evaluation of the safety of this folk medicine. |
| Databáze: | OpenAIRE |
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