Presence of Trimers in Glucagon Solution
| Autor: | J. Michael Creeth, Gilbert H. Beaven, Walter Gratzer |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Chemical Phenomena
Macromolecular Substances Hydrochloride Dimethyl Suberimidate Sodium chemistry.chemical_element Trimer Imides Guanidines Biochemistry chemistry.chemical_compound Methods Dicarboxylic Acids Guanidine Chromatography Sodium Dodecyl Sulfate Glucagon Molecular Weight Chemistry Electrophoresis Crystallography chemistry Sedimentation equilibrium Reagent Chromatography Gel Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Hydrochloric Acid Ultracentrifugation |
| Zdroj: | European Journal of Biochemistry. 31:505-509 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1972.tb02558.x |
| Popis: | Glucagon in solution at pH 10.2, at a concentration of 5 mg/ml, at which profiles of optical parameters against concentration show that it is substantially in its associated state, was covalently cross-linked with dimethyl suberimidate. At a suberimidate concentration of 1 mg/ml the formation of high-molecular-weight aggregates, which is observed at higher reagent concentrations, is suppressed, and a largely homogeneous product is eluted from a gel-filtration column in 6 M guanidine hydrochloride. This is also homogeneous as judged by polyacrylamide-gel electrophoresis in the presence of sodium dodecylsulphate. Sedimentation equilibrium in 6 M guanidine hydrochloride led to a molecular weight of about 9600, showing that the unique cross-linked form is the trimer; there is no evidence for dimers or hexamers, which have been inferred from sedimentation-equilibrium distributions. It is suggested that the trimeric form of high α-helix content may be equated with the rod-like trimers making up the asymmetric unit in the crystal. |
| Databáze: | OpenAIRE |
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