In silico designing of vaccine candidate against Clostridium difficile
| Autor: | Pratap D. Bade, Filippo Castiglione, Tamalika Kar, Anurag P. Srivastava, Utkarsh Narsaria, Debashrito Deb, Indraneel Sanyal, Srijita Basak |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
In silico Science Immunology 030106 microbiology Cloning vector Biology Major histocompatibility complex Biochemistry Article Microbiology 03 medical and health sciences Immune system Escherichia coli Humans vaccine designe Receptor Multidisciplinary Innate immune system Clostridioides difficile Computational Biology pipeline Clostridium difficile biology.organism_classification simulation molecular dynamics Computational biology and bioinformatics 030104 developmental biology in silico Bacterial Vaccines Clostridium Infections biology.protein Medicine Bacteria |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-22 (2021) Scientific reports (Nature Publishing Group) 11 (2021). doi:10.1038/s41598-021-93305-6 info:cnr-pdr/source/autori:Basak S.; Deb D.; Narsaria U.; Kar T.; Castiglione F.; Sanyal I.; Bade P.D.; Srivastava A.P./titolo:In silico designing of vaccine candidate against Clostridium difficile/doi:10.1038%2Fs41598-021-93305-6/rivista:Scientific reports (Nature Publishing Group)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-93305-6 |
| Popis: | Clostridium difficile is a spore-forming gram-positive bacterium, recognized as the primary cause of antibiotic-associated nosocomial diarrhoea. Clostridium difficile infection (CDI) has emerged as a major health-associated infection with increased incidence and hospitalization over the years with high mortality rates. Contamination and infection occur after ingestion of vegetative spores, which germinate in the gastro-intestinal tract. The surface layer protein and flagellar proteins are responsible for the bacterial colonization while the spore coat protein, is associated with spore colonization. Both these factors are the main concern of the recurrence of CDI in hospitalized patients. In this study, the CotE, SlpA and FliC proteins are chosen to form a multivalent, multi-epitopic, chimeric vaccine candidate using the immunoinformatics approach. The overall reliability of the candidate vaccine was validated in silico and the molecular dynamics simulation verified the stability of the vaccine designed. Docking studies showed stable vaccine interactions with Toll‐Like Receptors of innate immune cells and MHC receptors. In silico codon optimization of the vaccine and its insertion in the cloning vector indicates a competent expression of the modelled vaccine in E. coli expression system. An in silico immune simulation system evaluated the effectiveness of the candidate vaccine to trigger a protective immune response. |
| Databáze: | OpenAIRE |
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