Cardiac mesenchymal cells from failing and nonfailing hearts limit ventricular dilation when administered late after infarction
| Autor: | Yiru Guo, Yibing Nong, Steven P. Jones, Andrea Jurkovic, Xiaoping Zhu, Daniel W. Riggs, Marcin Wysoczynski, Tyler Weirick, Anna M. Gumpert, Alex Tomlin, Hong Li, Kenneth R. Brittian, Yi Wei Zheng, Shizuka Uchida, Bethany W. Long, Timothy N. Audam |
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| Rok vydání: | 2020 |
| Předmět: |
Cardiac function curve
musculoskeletal diseases Male medicine.medical_specialty Physiology Heart Ventricles Infarction Myocardial Reperfusion Injury macromolecular substances Mesenchymal Stem Cell Transplantation Cell therapy Mice Physiology (medical) Internal medicine medicine Animals Ventricular Function Myocytes Cardiac Myocardial infarction Ventricular remodeling Cells Cultured business.industry Mesenchymal stem cell technology industry and agriculture Mesenchymal Stem Cells medicine.disease Myocardial Contraction body regions Mice Inbred C57BL medicine.anatomical_structure Ventricle Heart failure Cardiology Female Cardiology and Cardiovascular Medicine business Transcriptome Research Article |
| Zdroj: | Am J Physiol Heart Circ Physiol |
| ISSN: | 1522-1539 |
| Popis: | Although cell therapy-mediated cardiac repair offers promise for treatment/management of heart failure, lack of fundamental understanding of how cell therapy works limits its translational potential. In particular, whether reparative cells from failing hearts differ from cells derived from nonfailing hearts remains unexplored. Here, we assessed differences between cardiac mesenchymal cells (CMC) derived from failing (HF) versus nonfailing (Sham) hearts and whether the source of donor cells (i.e., from HF vs. Sham) limits reparative capacity, particularly when administered late after infarction. To determine the impact of the donor source of CMCs, we characterized the transcriptional profile of CMCs isolated from sham (Sham-CMC) and failing (HF-CMC) hearts. RNA-seq analysis revealed unique transcriptional signatures in Sham-CMC and HF-CMC, suggesting that the donor source impacts CMC. To determine whether the donor source affects reparative potential, C57BL6/J female mice were subjected to 60 min of regional myocardial ischemia and then reperfused for 35 days. In a randomized, controlled, and blinded fashion, vehicle, HF-CMC, or Sham-CMC were injected into the lumen of the left ventricle at 35 days post-MI. An additional 5 weeks later, cardiac function was assessed by echocardiography, which indicated that delayed administration of Sham-CMC and HF-CMC attenuated ventricular dilation. We also determined whether Sham-CMC and HF-CMC treatments affected ventricular histopathology. Our data indicate that the donor source (nonfailing vs. failing hearts) affects certain aspects of CMC, and these insights may have implications for future studies. Our data indicate that delayed administration of CMC limits ventricular dilation and that the source of CMC may influence their reparative actions. NEW & NOTEWORTHY Most preclinical studies have used only cells from healthy, nonfailing hearts. Whether donor condition (i.e., heart failure) impacts cells used for cell therapy is not known. We directly tested whether donor condition impacted the reparative effects of cardiac mesenchymal cells in a chronic model of myocardial infarction. Although cells from failing hearts differed in multiple aspects, they retained the potential to limit ventricular remodeling. |
| Databáze: | OpenAIRE |
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