Mouse Fc??RI: identification and functional characterization of five new alleles
| Autor: | E. H. Leiter, A. L Gavin, P. M. Hogarth |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Mus spretus Molecular Sequence Data Immunology Mice Inbred Strains Mice Exon Mice Inbred NOD Sequence Homology Nucleic Acid Complementary DNA Genetics Animals Coding region Amino Acid Sequence Allele Gene Peptide sequence Alleles Mice Inbred BALB C COS cells Base Sequence Sequence Homology Amino Acid biology Receptors IgG biology.organism_classification Molecular biology COS Cells |
| Zdroj: | Immunogenetics. 51:206-211 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/s002510050033 |
| Popis: | The mouse Fcgr1 gene encoding the high-affinity IgG receptor (FcgammaRI) exists as two known alleles, FcgammaRI-BALB and FcgammaRI-NOD, and these alleles exhibit functional differences. To determine whether other alleles exist in mouse strains, Fcgr1 coding regions from 35 strains of mice were sequenced and a further five alleles were identified. The FcgammaRI-BALB and NOD alleles are now designated the "a" and "d" alleles, respectively. Analysis of the five new alleles revealed that although no polymorphisms were observed in the two leader exons, nucleotide and subsequent amino acid changes were observed in the exons encoding the extracellular domains, and transmembrane and cytoplasmic tail. The cDNA of the seven alleles (a-g) were isolated and transiently transfected into COS cells, and IgG-binding studies were performed. Receptors encoded by four of the five new alleles (b, c, f, g) bound IgG2a with high affinity, displaying IgG binding characteristics similar to the a allele (previously FcgammaRI-BALB). The d allele (previously FcgammaRI-NOD) and the e allele [derived from Mus spretus (SPRET/Ei)] encoded receptors which showed broader specificity by binding monomeric IgG2a, IgG2b, and IgG3. |
| Databáze: | OpenAIRE |
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