Assessment of fully automated antibody homology modeling protocols in molecular operating environment
| Autor: | Paul Labute, Johannes K. X. Maier |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Protein Conformation homology modeling loop refinement Immunoglobulin Variable Region Computational biology Biology loop enrichment Biochemistry Antibodies Hypervariable Loop comparative modeling Structural Biology Animals Humans Homology modeling Loop modeling Databases Protein Molecular Biology Genetics antibody engineering CDR-H3 loop modeling Operating environment Articles Complementarity Determining Regions Hypervariable region Template Fully automated Structural Homology Protein antibody structure biology.protein Antibody Software |
| Zdroj: | Proteins |
| ISSN: | 1097-0134 0887-3585 |
| Popis: | The success of antibody-based drugs has led to an increased demand for predictive computational tools to assist antibody engineering efforts surrounding the six hypervariable loop regions making up the antigen binding site. Accurate computational modeling of isolated protein loop regions can be quite difficult; consequently, modeling an antigen binding site that includes six loops is particularly challenging. In this work, we present a method for automatic modeling of the FV region of an immunoglobulin based upon the use of a precompiled antibody x-ray structure database, which serves as a source of framework and hypervariable region structural templates that are grafted together. We applied this method (on common desktop hardware) to the Second Antibody Modeling Assessment (AMA-II) target structures as well as an experimental specialized CDR-H3 loop modeling method. The results of the computational structure predictions will be presented and discussed. |
| Databáze: | OpenAIRE |
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