Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta
| Autor: | E. A. Vavilova, A. M. Chulkin, Olga S. Savinova, T. V. Tyazhelova, Tatiana V. Fedorova, Konstantin V. Moiseenko, Daria V. Vasina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
CAZy laccases lcsh:QR1-502 gene-tree/species-tree reconciliation Biology Trametes hirsuta Isozyme Genome Microbiology lcsh:Microbiology 03 medical and health sciences Phylogenetics evolution Polyporales Gene 030304 developmental biology Original Research Laccase 0303 health sciences 030306 microbiology biology.organism_classification phylogenetics isozymes Biochemistry polyporales |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| Popis: | Laccase is one of the oldest known and intensively studied fungal enzymes capable of oxidizing recalcitrant lignin-resembling phenolic compounds. It is currently well established that fungal genomes almost always contain several non-allelic copies of laccase genes (laccase multigene families); nevertheless, many aspects of laccase multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed evolutionary analysis of the sensu stricto laccase genes (CAZy - AA1_1) from fungi of the Polyporales order. The conducted analysis provides a better understanding of the Polyporales laccase multigenicity and allows for the systemization of the individual features of different laccase isozymes. In addition, we provide a comparison of the biochemical and catalytic properties of the four laccase isozymes from Trametes hirsuta and suggest their functional diversification within the multigene family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|