Mass spectrometric quantification of urinary 6-sulfatoxymelatonin

Autor: Anna van der Veen, Ido P. Kema, Martijn van Faassen, Sonja L. van Ockenburg, Helma de Jong, Elisabeth G.E. de Vries
Přispěvatelé: Guided Treatment in Optimal Selected Cancer Patients (GUTS), Lifestyle Medicine (LM)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Time Factors
Metabolite
Clinical Biochemistry
Physiology
melatonin
Urine
chemistry.chemical_compound
0302 clinical medicine
MELATONIN SECRETION
Solid phase extraction
Aged
80 and over
RISK
6-HYDROXYMELATONIN SULFATE
Biological Variation
Individual
medicine.diagnostic_test
Age Factors
General Medicine
Middle Aged
CANCER
urine
030220 oncology & carcinogenesis
Female
medicine.drug
Adult
Urinary system
Coefficient of variation
6-sulfatoxymelatonin
IMMUNOASSAY
Melatonin
Excretion
Young Adult
03 medical and health sciences
tandem mass spectrometry
medicine
Humans
RATES
Aged
biological variation
business.industry
Biochemistry (medical)
CIRCADIAN-RHYTHM
Reproducibility of Results
030104 developmental biology
SLEEP DISRUPTION
chemistry
Immunoassay
business
Chromatography
Liquid
Zdroj: Clinical chemistry and laboratory medicine, 59(1), 187-195. Walter de Gruyter GmbH
ISSN: 1434-6621
2020-0455
DOI: 10.1515/cclm-2020-0455
Popis: Objectives Regulators of circadian rhythm, including melatonin, influence fundamental biological processes. Measuring the melatonin metabolite 6-sulfatoxymelatonin in urine can estimate melatonin production. 6-sulfatoxymelatonin is mainly analyzed by immunoassays, but these methods are hampered by cross-reactivity and poor reproducibility when used to analyze small molecules. Therefore, we validated a high-throughput liquid chromatography with tandem mass spectrometry (LC–MS/MS) method to quantify 6-sulfatoxymelatonin in urine. We evaluated age-dependent 24-h excretion of 6-sulfatoxymelatonin into urine and the biological variation of urinary excretion in healthy individuals. Methods The online solid phase extraction method combined with LC–MS/MS was validated according to international guidelines, and used to measure the excretion of 6-sulfatoxymelatonin into urine of 240 healthy individuals. Biological variation of 6-sulfatoxymelatonin excretion was examined in 10 healthy individuals. Results Urinary 6-sulfatoxymelatonin results were well within the validation criteria (interassay coefficient of variation: Conclusions This MS-based method enables straightforward, reproducible, and sensitive quantification of 6-sulfatoxymelatonin in urine. Urinary 6-sulfatoxymelatonin levels decreased with age. Biological variation of 6-sulfatoxymelatonin excretion into urine was high between subjects and lower within subjects, indicating that repeated measurements of 6-sulfatoxymelatonin in 24-h urine are needed in future studies.
Databáze: OpenAIRE
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