Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
| Autor: | Alicia Fernández-San Millán, Inmaculada Farran, Camille Bénard, Iker Aranjuelo, Alisdair R. Fernie, María Ancín, Luis Larraya, Igor Florez-Sarasa, Yves Gibon, Jon Veramendi |
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| Přispěvatelé: | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. IMAB - Institute for Multidisciplinary Research in Applied Biology, Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación, Nafarroako Unibertsitate Publikoa. Agronomia, Bioteknologia eta Elikadura Saila, Producció Vegetal, Genòmica i Biotecnologia, Ministerio de Economía y Competitividad (España), Agence Nationale de la Recherche (France), European Commission, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
animal structures Chloroplasts Physiology Nitrogen Nitrogen assimilation Plant Science 01 natural sciences Chloroplast Glutamine synthetase GS-GOGAT pathway 03 medical and health sciences Chloroplast Thioredoxins Gene Expression Regulation Plant Tobacco Nitrogen metabolism Thioredoxin 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemistry AcademicSubjects/SCI01210 fungi Photorespiration Carbon metabolism food and beverages Metabolism Plants Genetically Modified Research Papers Carbon 3. Good health Amino acid Enzyme Biochemistry 010606 plant biology & botany Photosynthesis and Metabolism |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Journal of Experimental Botany Academica-e. Repositorio Institucional de la Universidad Pública de Navarra instname Digital.CSIC. Repositorio Institucional del CSIC IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| Popis: | In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase–glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. This work was supported by the Spanish Ministry of Science and Innovation (AGL2016-79868) and Phenome-FPPN (ANR-11-INBS-0012). MA is a recipient of a PhD fellowship from the Spanish Ministry of Education (FPU13/01675). IFS has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 753301, the ‘Ramon y Cajal’ contract RYC2019-027244-I/AEI/10.13039/501100011033, and the European Social Fund. |
| Databáze: | OpenAIRE |
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