Orthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography
| Autor: | E-Hu Liu, Ping Li, Yong Fan, Huijun Li, Raphael N. Alolga, Si-Hui Nian |
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| Rok vydání: | 2016 |
| Předmět: |
Anemarrhena
Chromatography Macroporous resin 010405 organic chemistry Chemistry 010401 analytical chemistry Filtration and Separation Area of interest Raw material 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Adsorption Column chromatography Neomangiferin Polyamide Organic chemistry Mangiferin Porosity Chromatography High Pressure Liquid Resins Plant Rhizome Drugs Chinese Herbal |
| Zdroj: | Journal of Separation Science. 39:3195-3204 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.201600274 |
| Popis: | The green and efficient preparation of natural products from biomass is considered an important area of interest in pharmaceutical applications. In this study, we aimed to provide a practical example with a popular traditional Chinese medicine, Anemarrhenae Rhizome, and showcase the orthogonal use of column chromatography with polyamide and macroporous adsorption resins to selectively concentrate and efficiently purify four bioactive compounds: neomangiferin, mangiferin, timosaponin B-II, and timosaponin A-III. Firstly, polyamide T60-100 was employed to fractionalize the crude extracts of Anemarrhenae Rhizome. Macroporous resin HPD400 was subsequently used to purify the xanthones and steroidal saponins. Under the optimized conditions, 2.31 g of neomangiferin, 4.10 g of mangiferin, 12.87 g of timosaponin B-II and 2.78 g of timosaponin A-III were prepared from 1 kg of crude materials, and their purities were 90.0, 92.15, 90.8 and 92.61%, respectively. The results of this study indicate that the combined column chromatography with polyamide and macroporous adsorption resins can be referenced as a green and efficient alternative for large-scale purification of bioactive ingredients from herbal raw materials. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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