MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
| Autor: | Jian-Kang Zhu, Nodoka Oka, Kenji Miura, Takuya Suzaki, Aiko Sato, Tsuyoshi Yamamoto, Na Renhu, Yasuomi Tada, Masaru Ohta |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Science Arabidopsis Biology 01 natural sciences Article 03 medical and health sciences Transcription (biology) Gene expression medicine Gene Transcription factor Multidisciplinary Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Cold-Shock Response Wild type biology.organism_classification Cell biology Cold Temperature 030104 developmental biology Medicine Cold sensitivity Signal transduction medicine.symptom Transcription Factors 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-29722-x |
| Popis: | The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid screening was performed. Two MYC-type transcription factors, MYC67 and MYC70, were identified as ICE1-interacting proteins. The myc mutants were more tolerant to freezing temperatures than wild type. CBF3/DREB1A and other cold-responsive genes were up-regulated in the myc mutants. Overexpression of the MYC genes increased the cold sensitivity and down-regulated the expression of cold-responsive genes. The MYC proteins interacted with the cis-elements in the CBF3/DREB1A promoter, probably to interfere interaction between ICE1 and the cis-elements. Taken together, these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance. |
| Databáze: | OpenAIRE |
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