Detrimental effects of 6 months exposure to very low doses of a mixture of six pesticides associated with chronic vitamin deficiency on rats
| Autor: | Antonio F. Hernández, Aristidis Tsatsakis, Marina D. Trebukh, N V Trusov, Daniela Calina, Ovidiu Zlatian, S.I. Shestakova, Guseva Gv, Lidiya V. Kravchenko, Oksana K. Mustafina, Anca Oana Docea, Marina Goumenou, Valentina A. Pashorina, Nadezhda V. Tyshko, Eleanora N. Trushina, Ludmila I. Avrenyeva, El'vira O. Sadykova, Nikolaj S. Nikitin, Valentin M. Zhminchenko, Maria S. Loginova |
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| Rok vydání: | 2021 |
| Předmět: |
Vitamin
Male Time Factors Physiology Urine Toxicology medicine.disease_cause Diquat 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology medicine Animals Pesticides Rats Wistar Triglycerides 030304 developmental biology 0303 health sciences medicine.diagnostic_test business.industry Body Weight Avitaminosis 04 agricultural and veterinary sciences General Medicine Lipase Organ Size Alkaline Phosphatase 040401 food science Urinary calcium Oxidative Stress Cholesterol chemistry Toxicity Chronic Disease Alkaline phosphatase Calcium Lipid profile business Oxidative stress Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 152 |
| ISSN: | 1873-6351 |
| Popis: | This study aimed to evaluate the long-term low-dose effects of exposure to a mixture of 6 pesticide active substances (diquat, imazamox, imazethapyr, tepraloxydin, bentazone, acifluorfen) and to elucidate if chronic vitamin deficiency can influence their toxicity. Two hundred Wistar rats were divided in 4 groups: a vitamin-sufficiency control group, a vitamin-deficiency control group, a vitamin sufficiency test group and a vitamin-deficiency test group. The test groups were treated with the aforementioned pesticides at doses 100 times lower than the corresponding NOAEL. After 6 months, ten rats from each group were sacrificed and a complete evaluation of blood and urine biochemistry, biomarkers of oxidative stress, xenobiotic detoxification enzymes and lysosomal enzymes and organ histopathology was performed. The pesticides mixture and vitamin deficiency determined an increase in alkaline phosphatase levels and urinary calcium levels, abnormal serum lipid profile, and a decrease of total blood proteins levels, red blood cells, haematocrit and haemoglobin. The combination of the two stressors up-regulated CYP1A1, CYP1A2, CYP2B1 and GST levels. This study provides a new proof for the need to move forward from single chemical testing to a more complex approach to account for the multitude of stressors that can challenge the setting of real safety levels. |
| Databáze: | OpenAIRE |
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