Regulated hyperacetylation of core histones during mouse spermatogenesis: involvement of histone deacetylases
| Autor: | M. Hazzouri, Christophe Pivot-Pajot, Yves Usson, A.-K. Faure, Sophie Rousseaux, B. Sèle, Saadi Khochbin, R. Pelletier |
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| Přispěvatelé: | Biologie moléculaire et cellulaire de la différenciation, Université Joseph Fourier - Grenoble 1 (UJF)-Institut Albert Bonniot-Institut National de la Santé et de la Recherche Médicale (INSERM), RFMQ, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF), IAB, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
endocrine system Histology medicine.drug_class Chromosomal Proteins Non-Histone [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Biology Histone Deacetylases Pathology and Forensic Medicine Histones Immunoenzyme Techniques Mice Histone H1 MESH: Chromosomal Proteins Non-Histone Proliferating Cell Nuclear Antigen Histone H2A Histone methylation Testis medicine Animals MESH: Animals Spermatogenesis MESH: Immunoenzyme Techniques MESH: Mice MESH: Histones urogenital system MESH: Testis Histone deacetylase inhibitor Acetylation Cell Biology General Medicine HDAC4 Molecular biology MESH: Male MESH: Histone Deacetylases Trichostatin A MESH: Proliferating Cell Nuclear Antigen Histone methyltransferase Histone deacetylase activity MESH: Spermatogenesis MESH: Acetylation medicine.drug |
| Zdroj: | European Journal of Cell Biology European Journal of Cell Biology, Elsevier, 2000, 79 (12), pp.950-60 |
| ISSN: | 0171-9335 |
| Popis: | Here we report a detailed analysis of waves of histone acetylation that occurs throughout spermatogenesis in mouse. Our data showed that spermatogonia and preleptotene spermatocytes contained acetylated core histones H2A, H2B and H4, whereas no acetylated histones were observed throughout meiosis in leptotene or pachytene spermatocytes. Histones remained unacetylated in most round spermatids. Acetylated forms of H2A and H2B, H3 and H4 reappeared in step 9 to 11 elongating spermatids, and disappeared later in condensing spermatids. The spatial distribution pattern of acetylated H4 within the spermatids nuclei, analyzed in 3D by immunofluorescence combined with confocal microscopy, showed a spatial sequence of events tightly associated with chromatin condensation. In order to gain an insight into mechanisms controlling histone hyperacetylation during spermiogenesis, we treated spermatogenic cells with a histone deacetylase inhibitor, trichostatin A (TSA), which showed a spectacular increase of histone acetylation in round spermatids. This observation suggests that deacetylases are responsible for maintaining a deacetylated state of histones in these cells. TSA treatment could not induce histone acetylation in condensing spermatids, suggesting that acetylated core histones are replaced by transition proteins without being previously deacetylated. Moreover, our data showed a dramatic decrease in histone deacetylases in condensing spermatids. Therefore, the regulation of histone deacetylase activity/concentration appears to play a major role in controling histone hyperacetylation and probably histone replacement during spermiogenesis. |
| Databáze: | OpenAIRE |
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