Effect of magnesium sulfate administration to improve insulin resistance in type 2 diabetes animal model: using the hyperinsulinemic-euglycemic clamp technique
| Autor: | Shahla Sohrabipour, Nepton Soltani, Mohammad Reza Sharifi, Ardeshir Talebi, Mohammadreza Sharifi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blood Glucose Male medicine.medical_specialty medicine.medical_treatment 030209 endocrinology & metabolism Diet High-Fat Glucagon Streptozocin Diabetes Mellitus Experimental 03 medical and health sciences Magnesium Sulfate 0302 clinical medicine Insulin resistance Internal medicine Diabetes mellitus medicine Animals Hypoglycemic Agents Insulin Pharmacology (medical) Rats Wistar Muscle Skeletal Pharmacology Glucose tolerance test Glucose Transporter Type 4 biology medicine.diagnostic_test Chemistry Insulin tolerance test Gluconeogenesis Glucose Tolerance Test medicine.disease Rats Disease Models Animal 030104 developmental biology Endocrinology Glucose Diabetes Mellitus Type 2 Liver biology.protein Glucose Clamp Technique Insulin Resistance Glucose 6-phosphatase GLUT4 |
| Zdroj: | Fundamentalclinical pharmacology. 32(6) |
| ISSN: | 1472-8206 |
| Popis: | This study attempted to elucidate the possible mechanism of magnesium sulfate (MgSO4 ) administration on reducing insulin resistance in type 2 diabetic rats. Fifty Wistar rats were divided into five groups: NDC was fed the normal diet, CD received high-fat diet with 35 mg/kg of streptozotocin, CD-Mg animals received MgSO4 via drinking water, CD-Ins1, and CD-Ins2 animals treated with low or high dose of insulin. Body weight and blood glucose levels were measured weekly. Intraperitoneal glucose tolerance test (IPGTT), insulin tolerance test, and metabolic cage assessment were performed monthly. After 12 weeks, the hyperinsulinemic-euglycemic clamp was performed for all animals and blood sample was taken to measure glycated hemoglobin (HbA1c), plasma insulin, glucagon, calcium, and magnesium levels. Liver and gastrocnemius muscle were isolated to measure glucagon receptor (GR), Glucose 6 phosphatase (G6Pase), Phosphoenolpyruvate carboxykinase (Pepck) and Glucose transporter 4 (Glut4) genes expression and GLUT4 protein translocation into the cell membrane. Consuming of high-fat diet generated insulin-resistant rats. Magnesium or insulin therapy altered insulin resistance, blood glucose, IPGTT, gluconeogenesis pathway, GR, body weight, the percentage of body fat, and HbA1C in diabetic rats. Administrations of MgSO4 or insulin in Type 2 diabetes mellitus animals increase GLUT4 gene and protein expression. Mg could improve glucose tolerance via stimulation of Glut4 gene expression and translocation and also suppression of the gluconeogenesis pathway and GR gene expression. Mg also increased glucose infusion rate and displayed beneficial effects in the treatment of glucose metabolism and improved insulin resistance. |
| Databáze: | OpenAIRE |
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