Presentation of functional groups on self-assembled supramolecular peptide nanofibers mimicking glycosaminoglycans for directed mesenchymal stem cell differentiation
| Autor: | Mustafa O. Guler, Oncay Yasa, Ayse B. Tekinay, Ozge Uysal, Melis Sardan Ekiz |
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| Přispěvatelé: | Yasa, Oncay, Uysal, Ozge, Ekiz, Melis Sardan, Güler, Mustafa O., Tekinay, Ayse B. |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Cells Nanofibers Biomedical Engineering Stem cells 02 engineering and technology Functional diversity 010402 general chemistry 01 natural sciences Organizational complexity Glycosaminoglycan Extracellular matrix Sulfation Carboxylation Peptide amphiphile General Materials Science Enzyme activity Extracellular matrices Peptide amphiphiles Cellular behaviors Glycoproteins Mesenchymal stem cell Tissue Developmental stage Proteins General Chemistry General Medicine 021001 nanoscience & nanotechnology Rats 0104 chemical sciences Cell biology Glucose Biochemistry Nanofiber Cell culture Mesenchymal stem cell differentiation Stem cell Cytology Peptides Supramolecular chemistry 0210 nano-technology Adipogenic differentiations |
| Zdroj: | Journal of Materials Chemistry B |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c7tb00708f |
| Popis: | Organizational complexity and functional diversity of the extracellular matrix regulate cellular behaviors. The extracellular matrix is composed of various proteins in the form of proteoglycans, glycoproteins, and nanofibers whose types and combinations change depending on the tissue type. Proteoglycans, which are proteins that are covalently attached to glycosaminoglycans, contribute to the complexity of the microenvironment of the cells. The sulfation degree of the glycosaminoglycans is an important and distinct feature at specific developmental stages and tissue types. Peptide amphiphile nanofibers can mimic natural glycosaminoglycans and/or proteoglycans, and they form a synthetic nanofibrous microenvironment where cells can proliferate and differentiate towards different lineages. In this study, peptide nanofibers were used to provide varying degrees of sulfonation mimicking the natural glycosaminoglycans by forming a microenvironment for the survival and differentiation of stem cells. The effects of glucose, carboxylate, and sulfonate groups on the peptide nanofibers were investigated by considering the changes in the differentiation profiles of rat mesenchymal stem cells in the absence of any specific differentiation inducers in the culture medium. The results showed that a higher sulfonate-to-glucose ratio is associated with adipogenic differentiation and a higher carboxylate-to-glucose ratio is associated with osteochondrogenic differentiation of the rat mesenchymal stem cells. Overall, these results demonstrate that supramolecular peptide nanosystems can be used to understand the fine-tunings of the extracellular matrix such as sulfation profile on specific cell types. © 2017 The Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
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