The Patatin-Containing Phospholipase A pPLAIIα Modulates Oxylipin Formation and Water Loss in Arabidopsis thaliana
| Autor: | Yueyun Hong, Wen-Yu Yang, Xuemin Wang, Mary R. Roth, Maoyin Li, Ruth Welti, Yong Zheng, Xiangqing Pan, Brad Scheu, Hieu Sy Vu, Sung Chul Bahn |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Hydrolases Linolenic acid Arabidopsis Cyclopentanes Plant Science Biology 01 natural sciences Phospholipases A Substrate Specificity Gene Knockout Techniques 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Oxylipins Abscisic acid Molecular Biology Research Articles 030304 developmental biology 0303 health sciences Phospholipase A Methyl jasmonate Arabidopsis Proteins Galactolipids Hydrolysis Jasmonic acid fungi Water Lysophosphatidylethanolamine food and beverages Oxylipin Droughts Up-Regulation Plant Leaves Phenotype Biochemistry chemistry Plant Stomata Lysophospholipids Patatin Oxidation-Reduction Abscisic Acid 010606 plant biology & botany |
| Zdroj: | Molecular Plant. 5(2):452-460 |
| ISSN: | 1674-2052 |
| DOI: | 10.1093/mp/ssr118 |
| Popis: | The patatin-related phospholipase A (pPLA) hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Ablation of pPLAIIα decreased lysophosphatidylcholine and lysophosphatidylethanolamine levels, but increased free linolenic acid. pPLAIIα-deficient plants displayed a higher level of jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid than wild-type (WT) plants. The expression of genes involved in oxylipin production was also higher in the pPLAIIα-deficient mutant than in WT plants. The mutant plants lost water more quickly than WT plants. The stomata of WT and mutant plants responded similarly to abscisic acid. In response to desiccation, the mutant and WT leaves produced abscisic acid at the same rate, but, after 4 h of desiccation, the jasmonic acid level was much higher in mutant than WT leaves. These results indicate that pPLAIIα negatively regulates oxylipin production and suggest a role in the removal of oxidatively modified fatty acids from membranes. |
| Databáze: | OpenAIRE |
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