C3a receptor modulation of granulocyte infiltration after murine focal cerebral ischemia is reperfusion dependent
| Autor: | William J. Mack, Ricardo J. Komotar, E. Sander Connolly, Zachary L. Hickman, Marc L. Otten, J. Mocco, Sergei A. Sosunov, Andrew F. Ducruet, Benjamin Hassid, David J Fusco, Grace H. Kim |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Anaphylatoxins Ischemia Granulocyte Arginine Brain Ischemia Brain ischemia Mice medicine Animals Anaphylatoxin Benzhydryl Compounds Receptor biology business.industry Brain Infarction Middle Cerebral Artery Intercellular adhesion molecule medicine.disease Flow Cytometry Intercellular Adhesion Molecule-1 Receptors Complement Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Neuroprotective Agents Neurology Reperfusion Injury biology.protein Complement C3a Neurology (clinical) C3a receptor Cardiology and Cardiovascular Medicine business Reperfusion injury Granulocytes |
| Zdroj: | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 28(5) |
| ISSN: | 0271-678X |
| Popis: | The complement anaphylatoxin C3a contributes to injury after cerebral ischemia in mice. This study assesses the effect of C3a receptor antagonist (C3aRA) on leukocyte infiltration into the ischemic zone. Transient or permanent middle cerebral artery occlusion (MCAO) was induced in wild-type C57BI/6 mice. Intraperitoneal C3aRA or vehicle was administered 45 mins before or 1 h after occlusion. Twenty-four hours after occlusion, we harvested brain tissue and purified inflammatory cells using flow cytometry. Soluble intercellular adhesion molecule (ICAM)-1 protein levels were assessed using enzyme-linked immunosorbent assays, and ICAM-1 and C3a receptor (C3aR) expression was confirmed via immunohistochemistry. In the transient MCAO model, animals receiving C3aRA showed smaller strokes, less upregulation of C3aR-positive granulocytes, and less ICAM-1 protein on endothelial cells than vehicle-treated animals; no significant differences in other inflammatory cell populations were observed. C3a receptor antagonist-treated and vehicle-treated animals showed no differences in stroke volume or inflammatory cell populations after permanent MCAO. These data suggest that blocking the binding of C3a to C3aR modulates tissue injury in reperfused stroke by inhibiting the recruitment of neutrophils to the ischemic zone. It further establishes antagonism of the C3a anaphylatoxin as a promising strategy for ameliorating injury after ischemia/reperfusion. |
| Databáze: | OpenAIRE |
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