Virus induces metal-binding proteins and changed trace element balance in the brain during the course of a common human infection (coxsackievirus B3) in mice
| Autor: | Peter Frisk, Nahla Mohamed, Inga-Lill Gadhasson, Jonas Blomberg, Nils-Gunnar Ilbäck, Göran Friman |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Environmental Engineering Ratón Coxsackievirus Infections Virus Replication Virus Mass Spectrometry Arsenic Mice Internal medicine Metals Heavy Gene expression medicine Environmental Chemistry Metallothionein Animals Waste Management and Disposal Cation Transport Proteins Regulation of gene expression Mice Inbred BALB C biology Brain Transporter DMT1 Mercury Pollution Metallothionein 3 Enterovirus B Human Zinc Endocrinology Viral replication Gene Expression Regulation Immunology biology.protein Female Copper |
| Zdroj: | The Science of the total environment. 381(1-3) |
| ISSN: | 0048-9697 |
| Popis: | Autopsy of the brain has shown a change in trace element balance in some virus-infected individuals, but it is not known whether this event was a result of the infection. In the present study coxsackievirus B3 (CVB3) adapted to Balb/c mice was used to study whether infection induces gene expression of the metal-binding/transporting proteins metallothionein (MT1 and MT3) and divalent-metal transporter 1 (DMT1) and whether it changes the balance of trace elements in the brain. Virus and MT1, MT3, and DMT1 were quantitatively measured by RT-PCR on days 3, 6 and 9 of the infection. Trace elements (13) were measured in serum and the brain by ICP-MS. High numbers of virus were found in the brain on days 3 and 6, but virus counts were decreased and present only in 50% of the mice on day 9. Gene expression of MT1 tended to increase on all days, whereas that of MT3 only showed a minor and not significant increase on day 3. No clear effect was observed in the expression of DMT1. The increase of MT3 was correlated to the brain concentration of Cu. The Cu/Zn ratio in serum increased as a response to the infection. There was a similar decrease in Cd in serum and the brain. On day 6 of the infection, Hg increased in the brain (p |
| Databáze: | OpenAIRE |
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