HP1BP3, a Chromatin Retention Factor for Co-transcriptional MicroRNA Processing
| Autor: | Masayuki Matsui, Kyoung Shin Yoo, Haoming Liu, Byung Cheon Jeong, Xiong Ke, Lisa N. Kinch, Zhiqiang Wang, Nick V. Grishin, David R. Corey, Yunsun Nam, Gaya P. Yadav, Rahul K. Kollipara, Chunyang Liang, Qinghua Liu, Ralf Kittler |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ribonuclease III Chromatin Immunoprecipitation Transcription Genetic Transfection Chromatin remodeling Article 03 medical and health sciences Histone H1 microRNA Animals Humans RNA Processing Post-Transcriptional Molecular Biology ChIA-PET Drosha Genetics Binding Sites biology Genome Human Nuclear Proteins RNA-Binding Proteins Cell Biology DNA Polymerase II Chromatin Cell biology DNA-Binding Proteins MicroRNAs 030104 developmental biology Histone Drosophila melanogaster biology.protein RNA Interference Chromatin immunoprecipitation HeLa Cells Protein Binding |
| Zdroj: | Molecular cell. 63(3) |
| ISSN: | 1097-4164 |
| Popis: | Recent studies suggest that the Microprocessor (Drosha-DGCR8) complex can be recruited to chromatin to catalyze co-transcriptional processing of primary microRNAs (pri-miRNAs) in mammalian cells. However, the molecular mechanism of co-transcriptional miRNA processing is poorly understood. Here, we find that HP1BP3, a histone H1-like chromatin protein, specifically associates with the Microprocessor and promotes global miRNA biogenesis in human cells. Chromatin immunoprecipitation (ChIP) studies reveal genome-wide co-localization of HP1BP3 and Drosha and HP1BP3-dependent Drosha binding to actively transcribed miRNA loci. Moreover, HP1BP3 specifically binds endogenous pri-miRNAs and facilitates the Drosha/pri-miRNA association in vivo. Knockdown of HP1BP3 compromises pri-miRNA processing by causing premature release of pri-miRNAs from the chromatin. Taken together, these studies suggest that HP1BP3 promotes co-transcriptional miRNA processing via chromatin retention of nascent pri-miRNA transcripts. This work significantly expands the functional repertoire of the H1 family of proteins and suggests the existence of chromatin retention factors for widespread co-transcriptional miRNA processing. |
| Databáze: | OpenAIRE |
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