Thermally-Responsive Loading and Release of Elastin-Like Polypeptides from Contact Lenses
| Autor: | Wan Wang, J. Andrew MacKay, Changrim Lee, Martha K. Pastuszka, Gordon W. Laurie |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
elastin-like polypeptide (ELPs)
genetic structures contact lens Pharmaceutical Science lcsh:RS1-441 Peptide protein therapeutics 02 engineering and technology Article lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound 030304 developmental biology chemistry.chemical_classification 0303 health sciences Protein therapeutics Coacervate Chemistry Reversible adsorption lacritin 021001 nanoscience & nanotechnology eye diseases Contact lens Drug delivery drug delivery Elastin like polypeptides sense organs 0210 nano-technology Ocular surface Biomedical engineering |
| Zdroj: | Pharmaceutics Volume 11 Issue 5 Pharmaceutics, Vol 11, Iss 5, p 221 (2019) |
| ISSN: | 1999-4923 |
| Popis: | Contact lenses are widely prescribed for vision correction, and as such they are an attractive platform for drug delivery to the anterior segment of the eye. This manuscript explores a novel strategy to drive the reversible adsorption of peptide-based therapeutics using commercially available contact lenses. To accomplish this, thermo-sensitive elastin-like polypeptides (ELPs) alone or tagged with a candidate ocular therapeutic were characterized. For the first time, this manuscript demonstrates that Proclear CompatiblesTM contact lenses are a suitable platform for ELP adsorption. Two rhodamine-labelled ELPs, V96 (thermo-sensitive) and S96 (thermo-insensitive), were employed to test temperature-dependent association to the contact lenses. During long-term release into solution, ELP coacervation significantly modulated the release profile whereby more than 80% of loaded V96 retained with a terminal half-life of ~4 months, which was only 1&ndash 4 days under solubilizing conditions. A selected ocular therapeutic candidate lacritin-V96 fusion (LV96), either free or lens-bound LV96, was successfully transferred to HCE-T cells. These data suggest that ELPs may be useful to control loading or release from certain formulations of contact lenses and present a potential for this platform to deliver a biologically active peptide to the ocular surface via contact lenses. |
| Databáze: | OpenAIRE |
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