Engineering CHO cells galactose metabolism to reduce lactate synthesis
| Autor: | Veronica Martinez, Natalia E Jiménez, Ziomara P. Gerdtzen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gene Expression Bioengineering CHO Cells 01 natural sciences Applied Microbiology and Biotechnology Galactokinase 03 medical and health sciences chemistry.chemical_compound Cricetulus 010608 biotechnology Animals Hexose Cell Engineering chemistry.chemical_classification Cell growth Chinese hamster ovary cell Galactose General Medicine Metabolism Transfection Carbon Recombinant Proteins 030104 developmental biology Biochemistry chemistry Cell culture Tissue Plasminogen Activator Lactates Biotechnology |
| Zdroj: | Biotechnology Letters. 41:779-788 |
| ISSN: | 1573-6776 0141-5492 |
| Popis: | Over-express galactokinase (Galk1) in tissue plasminogen activator (tPA) producing CHO cells as a potential strategy to improve cell growth and product synthesis. tPA producing CHO cells were transfected with the galactokinase (Galk1) gene. CHO-Galk1 cells showed a 39% increase of the specific growth rate in galactose. Moreover, clones were able to use this hexose as their main carbon source to sustain growth contrary to their parental cell line. Metabolic Flux Analysis revealed that the CHO-Galk1 selected clone shows an active metabolism towards biomass and product synthesis, characterized by higher fluxes in the TCA cycle, which is consistent with increased cellular densities and final product concentration. This cellular engineering strategy, where modifications of key points of alternative carbon sources metabolism lead to an improved metabolism of these sugars, is a starting point towards the generation of new cell lines with reduced lactate synthesis and increased cell growth and productivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink