Involvement of the Sinorhizobium meliloti leuA gene in activation of nodulation genes by NodD1 and luteolin
| Autor: | José Olivares, Socorro Muñoz, J. M. Sanjuan-Pinilla, Joaquina Nogales, Juan Sanjuán |
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| Rok vydání: | 2002 |
| Předmět: |
Transcriptional Activation
Rhizobiaceae Auxotrophy Mutant Plant Roots Biochemistry Microbiology chemistry.chemical_compound Bacterial Proteins Genetics Isomerases Luteolin Symbiosis Molecular Biology Gene Flavonoids Regulation of gene expression Sinorhizobium meliloti Models Genetic biology food and beverages General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification DNA-Binding Proteins Gene Expression Regulation chemistry Trans-Activators Leucine Medicago sativa Plasmids |
| Zdroj: | Archives of Microbiology. 178:36-44 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-002-0421-7 |
| Popis: | The role of leucine biosynthesis by Sinorhizobium meliloti in the establishment of nitrogen-fixing symbiosis with alfalfa ( Medicago sativa) was investigated. The leuA gene from S. meliloti, encoding alpha-isopropylmalate synthase, which catalyses the first specific step in the leucine biosynthetic pathway, was characterized. S. melilotiLeuA(-) mutants were Leu auxotrophs and lacked alpha-isopropylmalate synthase activity. In addition, leuA auxotrophs were unable to nodulate alfalfa. Alfalfa roots did not seem to secrete enough leucine to support growth of leucine auxotrophs in the rhizosphere. Thus, this growth limitation probably imposes the inability to initiate symbiosis. However, in addition to the leucine auxotrophy, leuA strains were impaired in activation of nodulation genes by the transcriptional activator NodD1 in response to the plant flavone luteolin. By contrast, nod gene activation by NodD3, which does not involve plant-derived inducers, was unaffected. Our results suggest that a leucine-related metabolic intermediate may be involved in activation of nodulation genes by NodD1 and luteolin. This kind of control could be of relevance as a way to link bacterial physiological status to the response to plant signals and initiation of symbiosis. |
| Databáze: | OpenAIRE |
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