Rapid topology probing using fluorescence spectroscopy in planar lipid bilayer: the pore-forming mechanism of the toxin Cry1Aa of Bacillus thuringiensis
| Autor: | Rikard Blunck, Marc Juteau, Nicolas Groulx |
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| Rok vydání: | 2010 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Insecticides Bacillus thuringiensis Toxins Physiology Chemistry Peripheral membrane protein Lipid Bilayers Bacillus thuringiensis Lipid bilayer fusion Biological membrane Membrane transport Topology Article Membrane Potentials Endotoxins Hemolysin Proteins Membrane Förster resonance energy transfer Spectrometry Fluorescence Bacterial Proteins Energy Transfer Membrane fluidity Animals Lipid bilayer Fluorescent Dyes |
| Zdroj: | The Journal of General Physiology |
| ISSN: | 1540-7748 |
| Popis: | Pore-forming toxins, many of which are pathogenic to humans, are highly dynamic proteins that adopt a different conformation in aqueous solution than in the lipid environment of the host membrane. Consequently, their crystal structures obtained in aqueous environment do not reflect the active conformation in the membrane, making it difficult to deduce the molecular determinants responsible for pore formation. To obtain structural information directly in the membrane, we introduce a fluorescence technique to probe the native topology of pore-forming toxins in planar lipid bilayers and follow their movement during pore formation. Using a Förster resonance energy transfer (FRET) approach between site-directedly labeled proteins and an absorbing compound (dipicrylamine) in the membrane, we simultaneously recorded the electrical current and fluorescence emission in horizontal planar lipid bilayers formed in plastic chips. With this system, we mapped the topology of the pore-forming domain of Cry1Aa, a biological pesticide from Bacillus thuringiensis, by determining the location of the loops between its seven α helices. We found that the majority of the toxins initially traverse from the cis to the trans leaflet of the membrane. Comparing the topologies of Cry1Aa in the active and inactive state in order to identify the pore-forming mechanism, we established that only the α3–α4 hairpin translocates through the membrane from the trans to the cis leaflet, whereas all other positions remained constant. As toxins are highly dynamic proteins, populations that differ in conformation might be present simultaneously. To test the presence of different populations, we designed double-FRET experiments, where a single donor interacts with two acceptors with very different kinetics (dipicrylamine and oxonol). Due to the nonlinear response of FRET and the dynamic change of the acceptor distribution, we can deduce the distribution of the acceptors in the membrane from the time course of the donor fluorescence. We found that Cry1Aa is present on both membrane leaflets. |
| Databáze: | OpenAIRE |
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