Ultrastructural analysis of the dehydrated tardigrade Hypsibius exemplaris unveils an anhydrobiotic-specific architecture

Autor: Yoshihisa Mori, Simon Galas, Chantal Cazevielle, Nelly Godefroy, Fumihisa Ono, Emilie Le Goff, Naurang L. Saini, Stephen Baghdiguian, Pierre Cuq, Myriam Richaud
Přispěvatelé: Bodescot, Myriam, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Okayama University of Science, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), The present study was supported by the CNRS 'Défi Origines 2018' – Project GigaTardi (grant no. 265880 – Giga18)., Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Plateau MRI-COMET [Montpellier], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Institut des Neurosciences de Montpellier (INM), Università degli Studi di Roma 'La Sapienza' [Rome], École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome]
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
tardigrade
Cell
ultrastructural analysis
lcsh:Medicine
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Mitochondrion
Cellular imaging
Article
03 medical and health sciences
Microscopy
Electron
Transmission
Organelle
medicine
Tardigrada
Electron microscopy
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Animals
Cellular organization
Desiccation
lcsh:Science
Cell Nucleus
Multidisciplinary
030102 biochemistry & molecular biology
biology
Chemistry
Endoplasmic reticulum
lcsh:R
biology.organism_classification
Adaptation
Physiological
Cell biology
Mitochondria
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
030104 developmental biology
medicine.anatomical_structure
tardigrade
ultrastructural analysis
morphological features
morphological features
Ultrastructure
[SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
lcsh:Q
Tardigrade
Hypsibius
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports
Scientific Reports, 2020, 10 (1), pp.4324. ⟨10.1038/s41598-020-61165-1⟩
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.4324. ⟨10.1038/s41598-020-61165-1⟩
ISSN: 2045-2322
DOI: 10.1038/s41598-020-61165-1
Popis: Tardigrades can cope with adverse environmental conditions by turning into anhydrobiotes with a characteristic tun shape. Tun formation is an essential morphological adaptation for tardigrade entry into the anhydrobiotic state. The tun cell structure and ultrastructure have rarely been explored in tardigrades in general and never in Hypsibius exemplaris. We used transmission electron microscopy to compare cellular organization and ultrastructures between hydrated and anhydrobiotic H. exemplaris. Despite a globally similar cell organelle structure and a number of cells not significantly different between hydrated and desiccated tardigrades, reductions in the sizes of both cells and mitochondria were detected in dehydrated animals. Moreover, in anhydrobiotes, secretory active cells with a dense endoplasmic reticulum network were observed. Interestingly, these anhydrobiote-specific cells are in a close relationship with a specific extracellular structure surrounding each cell. It is possible that this rampart-like extracellular structure resulted from the accumulation of anhydrobiotic-specific material to protect the cells. Interestingly, after five hours of rehydration, the number of secretory cells decreased, and the specific extracellular structure began to disappear. Twenty-four hours after the beginning of rehydration, the cellular structure and ultrastructure were comparable to those observed in hydrated tardigrades.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje