RNA-Seq Analysis of the Sclerotinia homoeocarpa – Creeping Bentgrass Pathosystem
| Autor: | Jinnan Hu, Angela M. Orshinsky, Michael J. Boehm, Venu Reddyvari-Channarayappa, Thomas K. Mitchell, Stephen O. Opiyo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Dollar spot
Antifungal Agents Time Factors Plant Pathogens lcsh:Medicine Virulence Gene Expression RNA-Seq Plant Science Microbiology Agrostis Lignin Transcriptomes Molecular Genetics Pathosystem Plant Microbiology Ascomycota Genome Analysis Tools Gene Expression Regulation Plant Gene Expression Regulation Fungal Plant defense against herbivory lcsh:Science Biology N-Glycosyl Hydrolases Gene Library Genetics Plant Growth and Development Multidisciplinary biology Models Genetic Sequence Analysis RNA lcsh:R Computational Biology Chromosome Mapping Genomics Sequence Analysis DNA Plant Pathology biology.organism_classification Drug Resistance Multiple Protein Structure Tertiary Fungicide RNA lcsh:Q Genome Expression Analysis Software Research Article Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 8, p e41150 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Sclerotinia homoeocarpa causes dollar spot disease, the predominate disease on highly-maintained turfgrass. Currently, there are major gaps in our understanding of the molecular interactions between S. homoeocarpa and creeping bentgrass. In this study, 454 sequencing technology was used in the de novo assembly of S. homoeocarpa and creeping bentgrass transcriptomes. Transcript sequence data obtained using Illumina's first generation sequencing-by-synthesis (SBS) were mapped to the transcriptome assemblies to estimate transcript representation in different SBS libraries. SBS libraries included a S. homoeocarpa culture control, a creeping bentgrass uninoculated control, and a library for creeping bentgrass inoculated with S. homoeocarpa and incubated for 96 h. A Fisher's exact test was performed to determine transcripts that were significantly different during creeping bentgrass infection with S. homoeocarpa. Fungal transcripts of interest included glycosyl hydrolases, proteases, and ABC transporters. Of particular interest were the large number of glycosyl hydrolase transcripts that target a wide range of plant cell wall compounds, corroborating the suggested wide host range and saprophytic abilities of S. homoeocarpa. Several of the multidrug resistance ABC transporters may be important for resistance to both fungicides and plant defense compounds. Creeping bentgrass transcripts of interest included germins, ubiquitin transcripts involved in proteasome degradation, and cinnamoyl reductase, which is involved in lignin production. This analysis provides an extensive overview of the S. homoeocarpa-turfgrass pathosystem and provides a starting point for the characterization of potential virulence factors and host defense responses. In particular, determination of important host defense responses may assist in the development of highly resistant creeping bentgrass varieties. |
| Databáze: | OpenAIRE |
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