Small ruminant lentivirus infection influences expression of acute phase proteins and cathelicidin genes in milk somatic cells and peripheral blood leukocytes of dairy goats

Autor: Lech Zwierzchowski, Michał Czopowicz, Daria Reczyńska, Magdalena Zalewska, Emilia Bagnicka, Jarosław Kaba
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
040301 veterinary sciences
Somatic cell
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
animal diseases
Enzyme-Linked Immunosorbent Assay
Biology
Real-Time Polymerase Chain Reaction
Cathelicidin
Cathelicidin Gene
0403 veterinary science
Andrology
03 medical and health sciences
Immune system
Blood serum
Milk Somatic Cell (MSC)
Infected Goats
Cathelicidins
medicine
Leukocytes
Uninfected Goats
Animals
2. Zero hunger
Messenger RNA
Goat Diseases
lcsh:Veterinary medicine
General Veterinary
Small Ruminant Lentiviruses (SRLV)
Goats
Acute-phase protein
Decreased Concentration
04 agricultural and veterinary sciences
biology.organism_classification
3. Good health
stomatognathic diseases
030104 developmental biology
Milk
Lentivirus
Lentivirus Infections
lcsh:SF600-1100
Research Article
Acute-Phase Proteins
Antimicrobial Cationic Peptides
Zdroj: Veterinary Research, Vol 49, Iss 1, Pp 1-13 (2018)
Veterinary Research
Veterinary Research, BioMed Central, 2018, 49 (1), pp.113. ⟨10.1186/s13567-018-0607-x⟩
ISSN: 1297-9716
0928-4249
Popis: International audience; AbstractThe aim of the study was to analyze acute phase protein and cathelicidin gene responses to small ruminant lentivirus (SRLV) infection in goats. In uninfected goats, we found higher Cp and lower Fbγ mRNA levels in blood leucocytes (BL) than in milk somatic cells (MSC), as well as lower SAA, Hp, and CRP and higher Cp and AGP concentrations in blood serum than in milk. In SRLV-infected goats, we found higher Fbγ and MAP28 and lower Cp expression in MSC than in BL, and higher SAA, Hp, Fb, and MAP28 and lower AGP concentrations in milk than in blood serum. Higher SAA and Hp expressions in BL and Hp expression in MSC were found in SRLV-infected goats. In SRLV-infected goats, we observed a higher concentration of SAA in blood serum, while in milk, lower SAA, Cp, and MAP28 and higher MAP34 concentrations were observed. The expression profiles of the studied genes differed between BL/serum and MSC/milk. The elevated SAA concentration in blood serum was accompanied by a decreased concentration of SAA and Cp in the milk of infected goats. No differences in the expression of the other studied genes may mean that the SRLV has the ability to evade the immune system, continuing to replicate. The elevated concentration of SAA in blood serum may promote viral multiplication. This higher concentration of SAA in blood serum and simultaneous reduced concentration of SAA and Cp in milk may be additive indicators of this infection.
Databáze: OpenAIRE
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